抗大肠杆菌O4脂多糖和H5鞭毛蛋白的鼠单克隆抗体。
Murine monoclonal antibodies against Escherichia coli O4 lipopolysaccharide and H5 flagellin.
作者信息
Rivera-Betancourt M, Keen J E
机构信息
United States Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933, USA.
出版信息
J Clin Microbiol. 2001 Sep;39(9):3409-13. doi: 10.1128/JCM.39.9.3409-3413.2001.
Abstract
Two murine monoclonal antibodies (MAb), 2C5-F10 and 8D1-H10, reactive with Escherichia coli O4 and H5 antigens, respectively, were generated and characterized. Enzyme immunoassays and immunoblots demonstrated that MAb 2C5-F10 reacted specifically with lipopolysaccharide O antigen of E. coli O4 isolates, while MAb 8D1-H10 reacted with E. coli strains expressing H5 flagella.
摘要
产生并鉴定了两种分别与大肠杆菌O4和H5抗原反应的鼠单克隆抗体（MAb），即2C5-F10和8D1-H10。酶免疫测定和免疫印迹表明，单克隆抗体2C5-F10与大肠杆菌O4分离株的脂多糖O抗原特异性反应，而单克隆抗体8D1-H10与表达H5鞭毛的大肠杆菌菌株反应。
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