东亚钳蝎重组α-昆虫毒素rBmKalphaIT1的结晶及初步晶体学研究

Crystallization and preliminary crystallographic study of rBmKalphaIT1, a recombinant alpha-insect toxin from the scorpion Buthus martensii Karsch.

作者信息

Huang Ying, Huang Qichen, Chen Haibao, Tang Youqi, Miyake Hideo, Kusunoki Masami

机构信息

Department of Chemical Biology, College of Chemistry, Peking University, Beijing 100871, People's Republic of China.

出版信息

Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1635-6. doi: 10.1107/s090744490301415x. Epub 2003 Aug 19.

DOI:10.1107/s090744490301415x

PMID:12925796

Abstract

Alpha-insect scorpion toxins are a distinct group of scorpion neurotoxins for which no crystal structures are yet available. A novel alpha-insect toxin named BmKalphaIT1 from the scorpion Buthus martensii Karsch (BmK) has been expressed as an inclusion body in Escherichia coli and purified by chromatography after renaturation. Recombinant BmKalphaIT1 (rBmKaIT1) was crystallized using the vapour-diffusion technique in hanging drops at 296 K. The crystals, which were grown in sodium phosphate, belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 30.24 (1), b = 36.51 (3), c = 57.08 (2) A. Diffraction data were collected to 2.1 A resolution using synchrotron radiation. There appears to be one rBmKalphaIT1 molecule in the asymmetric unit.

摘要

α-昆虫蝎毒素是一类独特的蝎神经毒素，目前尚无其晶体结构。一种名为BmKαIT1的新型α-昆虫毒素，从东亚钳蝎（BmK）中分离得到，在大肠杆菌中以包涵体形式表达，复性后经色谱法纯化。重组BmKαIT1（rBmKaIT1）采用气相扩散技术在296 K的悬滴中结晶。在磷酸钠中生长的晶体属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数a = 30.24 (1)，b = 36.51 (3)，c = 57.08 (2) Å。使用同步辐射收集衍射数据至2.1 Å分辨率。不对称单元中似乎有一个rBmKαIT1分子。