Gayet J, Zhou X P, Duval A, Rolland S, Hoang J M, Cottu P, Hamelin R
INSERM U434 - CEPH, Paris, France.
Oncogene. 2001 Aug 16;20(36):5025-32. doi: 10.1038/sj.onc.1204611.
A number of genetic alterations have been described in colorectal cancers. They include allelic losses on specific chromosomal arms, mutations of oncogenes, tumor suppressor genes and mismatch repair genes, microsatellite instability in coding repeat sequences of target genes and methylation defects in gene promoters. Since these alterations have been reported by different groups on different tumors and cell lines, the complete repertoire of genetic alterations for any given tumor sample remains unknown. In the present study, we analysed a series of 22 colorectal cancer cell lines for 31 different genetic alterations. We found significant correlations between mutational profiles in these colorectal cell lines associated with differences in mismatch repair status. This panel of colon cancer cell lines is representative of the genetic heterogeneity occurring in sporadic colorectal carcinoma. Our results may prove to be very useful for understanding the different biological pathways involved in the development of colon cancer, and for groups studying cellular biology and pharmacology on the same cell lines.
在结直肠癌中已描述了多种基因改变。它们包括特定染色体臂上的等位基因缺失、癌基因、肿瘤抑制基因和错配修复基因的突变、靶基因编码重复序列中的微卫星不稳定性以及基因启动子中的甲基化缺陷。由于不同研究小组在不同肿瘤和细胞系中报告了这些改变，任何给定肿瘤样本的完整基因改变情况仍然未知。在本研究中，我们分析了一系列22个结直肠癌细胞系中的31种不同基因改变。我们发现这些结直肠癌细胞系中的突变谱与错配修复状态的差异之间存在显著相关性。这组结肠癌细胞系代表了散发性结直肠癌中发生的基因异质性。我们的结果可能对理解结肠癌发生过程中涉及的不同生物学途径以及对在同一细胞系上研究细胞生物学和药理学的研究小组非常有用。
