Lee C H, Jung H S, Lee T Y, Lee S R, Yuk S W, Lee K G, Lee B H
Department of Anatomy and Meridianology, College of Oriental Medicine, Woosuk University, Cheju, Korea.
Am J Chin Med. 2001;29(2):211-20. doi: 10.1142/S0192415X01000241.
The purpose of this morphological study was to investigate the relation between the meridian, meridian points and viscera using neuroanatomical tracers. The common locations of the spinal cord and brain projecting to the stomach and Zusanli were observed following injection of CTB (cholera toxin B subunit) and pseudorabies viruses (PRV-Ba, Bartha strain and PRV-Ba-Gal, galactosidase insertion) into the stomach and Zusanli (ST36). After 4-5 days of survival following injection into twelve rats, they were perfused, and their spinal cords and brains were frozen sectioned (30 microm). These sections were stained by X-gal histochemical, CTB and PRV-Ba immunohistochemical staining methods, and examined with the light microscope. The results were as follows: Commonly labeled medulla oblongata regions were dorsal motor nucleus of vagus nerve (DMV), nucleus tractus solitarius (NTS) and area postrema (AP) following injection of CTB and PRV-Ba-Gal into stomach and Zusanli, respectively. In the spinal cord, commonly labeled neurons were found in thoracic, lumbar and sacral spinal segments. Densely labeled areas were found in lamina IV, V, VII (intermediolateral nucleus) and X of the spinal cord. In the brain, commonly labeled neurons were found in the Al noradrenalin cells/Cl adrenalin cells/caudoventrolateral reticular nucleus, dorsal motor nucleus of vagus nerve, nucleus tractus solitarius, area postrema, raphe obscurus nucleus, raphe pallidus nucleus, raphe magnus nucleus, gigantocellular nucleus, locus coeruleus, parabrachial nucleus, Kolliker-Fuse nucleus, A5 cell group, central gray matter, paraventricular hypothalamic nucleus, lateral hypothalamic nucleus, retrochiasmatic hypothalamic nucleus, bed nucleus of stria terminalis and amygdaloid nucleus. Thus central autonomic center project both to the stomach and Zusanli. These morphological results suggest that there is a commonality of CNS cell groups in brain controlling stomach (viscera) and Zusanli (limb).
本形态学研究的目的是使用神经解剖示踪剂研究经络、穴位与脏腑之间的关系。将霍乱毒素B亚基（CTB）和伪狂犬病病毒（PRV-Ba，Bartha株以及PRV-Ba-Gal，插入半乳糖苷酶）注入胃和足三里（ST36）后，观察投射至胃和足三里的脊髓和脑的常见部位。向12只大鼠注射后存活4至5天，对其进行灌注，然后将其脊髓和脑制成冰冻切片（30微米）。这些切片采用X-gal组织化学、CTB和PRV-Ba免疫组织化学染色方法进行染色，并在光学显微镜下检查。结果如下：分别向胃和足三里注射CTB和PRV-Ba-Gal后，延髓的常见标记区域为迷走神经背运动核（DMV）、孤束核（NTS）和最后区（AP）。在脊髓中，在胸段、腰段和骶段脊髓节段发现了常见标记的神经元。在脊髓的IV层、V层、VII层（中间外侧核）和X层发现了密集标记区域。在脑中，在去甲肾上腺素能A1细胞/肾上腺素能C1细胞/尾侧腹外侧网状核、迷走神经背运动核、孤束核、最后区、中缝隐核、中缝苍白核、中缝大核、巨细胞网状核、蓝斑、臂旁核、 Kölliker-Fuse核、A5细胞群、中央灰质、下丘脑室旁核、下丘脑外侧核、视交叉后下丘脑核、终纹床核和杏仁核中发现了常见标记的神经元。因此，中枢自主神经中枢同时投射至胃和足三里。这些形态学结果表明，在脑内控制胃（脏腑）和足三里（肢体）的中枢神经系统细胞群存在共性。
