Vizzard M A, Erickson V L, Card J P, Roppolo J R, de Groat W C
Department of Pharmacology, University of Pittsburgh, Pennsylvania 15261, USA.
J Comp Neurol. 1995 May 15;355(4):629-40. doi: 10.1002/cne.903550411.
Transneuronal tracing techniques were used to identify sites in the central nervous system involved in the neural control of urethral function. The distribution of virus-infected neurons was examined in the spinal cord and brainstem at various intervals (56-96 hours) following pseudorabies virus (PRV) injection into the urethra. In the lumbosacral (L6-S1) spinal cord at 56 hours, neurons containing PRV immunoreactivity (PRV-IR) were located in the region of the sacral parasympathetic nucleus (SPN), around the central canal, and in the dorsal commissure. Some animals also exhibited PRV-IR in cells in the L6 dorsolateral motor nucleus. At longer survival times (72-96 hours), PRV-IR cells were observed in the superficial and deeper laminae of the dorsal horn, and increased numbers of PRV-IR cells were consistently detected in the region of the SPN, around the central canal, and in the dorsal commissure. PRV-IR fiber-like staining also occurred along the lateral edge of the dorsal horn extending from Lissauer's tract to the region of the SPN. In rostral lumbar segments (L1-L2), PRV-IR cells were located in the region of the dorsal commissure and the intermediolateral cell nucleus (IML), around the central canal, and in the dorsal horn. After 72-84 hours, PRV-IR cells were also noted at more rostral levels of the neuraxis including the medulla, pons, midbrain, and diencephalon. At 72 hours, PRV-IR cells were consistently observed in Barrington's nucleus (pontine micturition center), nucleus raphe magnus (RMg), parapyramidal reticular formation, and the A5 and A7 regions. At 78-84 hours, additional regions exhibited PRV-IR cells, including the periaqueductal gray, locus coeruleus, the dorsal and ventral subcoeruleus alpha, and the red nucleus. A few cells were also located in the lateral hypothalamic area. This distribution of PRV-labeled cells in the spinal cord and brainstem is similar in many respects to the distribution of cells labeled in previous studies by PRV injection into the urinary bladder. This overlap of urethra and bladder neurons is consistent with the results of physiological experiments indicating a close coordination between the central nervous control of bladder and urethral activity.
运用跨神经元追踪技术来确定中枢神经系统中参与尿道功能神经控制的部位。在将伪狂犬病病毒（PRV）注入尿道后的不同时间间隔（56 - 96小时），对脊髓和脑干中病毒感染神经元的分布进行了检查。在56小时时，腰荐部（L6 - S1）脊髓中，含有PRV免疫反应性（PRV - IR）的神经元位于骶副交感核（SPN）区域、中央管周围以及背侧连合。一些动物的L6背外侧运动核中的细胞也显示出PRV - IR。在更长的存活时间（72 - 96小时），在背角的浅层和深层板层中观察到PRV - IR细胞，并且在SPN区域、中央管周围以及背侧连合中持续检测到数量增加的PRV - IR细胞。PRV - IR纤维样染色也沿着从Lissauer束延伸至SPN区域的背角外侧边缘出现。在腰段上部（L1 - L2），PRV - IR细胞位于背侧连合区域、中间外侧细胞核（IML）、中央管周围以及背角。72 - 84小时后，在包括延髓、脑桥、中脑和间脑在内的更靠头端的神经轴水平也发现了PRV - IR细胞。在72小时时，在巴林顿核（脑桥排尿中枢）、中缝大核（RMg）、锥体旁网状结构以及A5和A7区域持续观察到PRV - IR细胞。在78 - 84小时，其他区域也出现了PRV - IR细胞，包括导水管周围灰质、蓝斑、背侧和腹侧蓝斑下α以及红核。少数细胞也位于下丘脑外侧区。脊髓和脑干中PRV标记细胞的这种分布在许多方面与先前通过将PRV注入膀胱的研究中标记的细胞分布相似。尿道和膀胱神经元的这种重叠与生理实验结果一致，表明膀胱和尿道活动的中枢神经控制之间存在密切协调。
