啮目昆虫（书虱）：孟买一种新的主要室内过敏原。

Psocoptera spp. (book louse): a new major household allergen in Mumbai.

作者信息

Patil M P, Niphadkar P V, Bapat M M

机构信息

The Institute of Science, Mumbai, India.

出版信息

Ann Allergy Asthma Immunol. 2001 Aug;87(2):151-5. doi: 10.1016/S1081-1206(10)62211-9.

DOI:10.1016/S1081-1206(10)62211-9

PMID:11527249

Abstract

BACKGROUND

Exposure and sensitization to indoor allergens of house dust mites and insects is well established. However, there are no reports on the allergenicity of Psocoptera spp. (book louse). These insects are known to inhabit various terrestrial habitats such as tree bark (bark lice), old papers, books, stored food products, etc and gain entry into human dwellings through these modes. They have the potential to sensitize individuals with an occupational exposure to them.

OBJECTIVE

Our objective is to use clinical and immunologic techniques to evaluate the extent of sensitization to Psocoptera in patients with nasobronchial allergy. We also have the objective to determine the extent of Psocoptera infestation in houses in Mumbai (Bombay).

METHODS

An entomologic analysis of dust samples in houses in Mumbai was carried out and Psocoptera found in these samples was mass-cultured. Two hundred patients were checked for allergy to the Psocoptera extracts using skin test. Total IgE and specific IgE to Psocoptera in sera from Psocopotera-sensitive patients' sera were estimated by enzyme immunoassay. IgE-binding proteins of Psocoptera were determined by immunoblotting experiments.

RESULTS

Among the 75 house dust samples from Mumbai analyzed for the presence of Psocoptera, 25% were found to be infested by Psocoptera. Strong skin sensitivity to Psocoptera was found in 20% of patients. Total IgE levels >150 IU/mL were found in these patients and high levels of specific IgE to Psocoptera were detected by ELISA. Immunoblot analysis revealed 67, 59, 43, and 27 kD proteins as major allergens of Psocoptera.

CONCLUSIONS

Psocoptera is an important allergen in Mumbai as it causes IgE mediated responses in 20% of the allergic population studied.

摘要

背景

对屋尘螨和昆虫等室内过敏原的暴露及致敏作用已得到充分证实。然而，关于啮目昆虫（书虱）的致敏性尚无相关报道。已知这些昆虫栖息于各种陆地生境，如树皮（树虱）、旧纸张、书籍、储存的食品等，并通过这些途径进入人类住所。它们有可能使职业接触者产生致敏反应。

目的

我们的目的是运用临床和免疫学技术评估鼻支气管过敏患者对啮目昆虫的致敏程度。我们还旨在确定孟买（旧称： Bombay）房屋中啮目昆虫的侵扰程度。

方法

对孟买房屋中的灰尘样本进行了昆虫学分析，并对这些样本中发现的啮目昆虫进行了大规模培养。使用皮肤试验对200名患者进行了对啮目昆虫提取物过敏的检查。通过酶免疫测定法估计了对啮目昆虫敏感患者血清中总IgE和特异性IgE的水平。通过免疫印迹实验确定了啮目昆虫的IgE结合蛋白。

结果

在对来自孟买的75份房屋灰尘样本进行的啮目昆虫存在情况分析中，发现25%的样本受到啮目昆虫侵扰。20%的患者对啮目昆虫表现出强烈的皮肤敏感性。这些患者的总IgE水平>150 IU/mL，并且通过ELISA检测到高水平的对啮目昆虫的特异性IgE。免疫印迹分析显示67、59、43和27 kD蛋白是啮目昆虫的主要过敏原。