Baldus S E, Zirbes T K, Glossmann J, Fromm S, Hanisch F G, Mönig S P, Schröder W, Schneider P M, Flucke U, Karsten U, Thiele J, Hölscher A H, Dienes H P
Institute of Pathology, University of Cologne, Germany.
Oncology. 2001;61(2):147-55. doi: 10.1159/000055366.
The Thomsen-Friedenreich (TF) antigen is a well-known human pan-carcinoma antigen. It represents a carbohydrate core disaccharide (Gal beta 1-3GalNAc) which is predominantly bound to mucin peptide cores. Its immunoreactivity depends on changes in glycosylation which lead to a reduction in the carbohydrate chain length and the exposure of core carbohydrates. In the present study, we investigated 208 gastric adenocarcinomas with respect to their immunohistochemical reactivity applying two monoclonal antibodies (MAbs). MAb specifically detecting TF antigen (A78-G/A7) and MAb BW835 were included. The latter reacts with a certain glycoform of the MUC1 peptide core, characterized by core-type glycans like TF. A78-G/A7 epitopes were detected in 68.8% and BW835 epitopes in 57.7% of the carcinomas. BW835 immunoreactivity correlated with the presence of lymph node metastases. Both A78-G/A7 and BW835 staining were significantly stronger in tubular/papillary cancer (WHO classification) and intestinal-type cancer according to Laurén. In univariate survival analyses of all patients studied, BW835 immunoreactivity was a marker of an unfavorable prognosis (p < 0.05). The presence of A78-G/A7 and BW835 epitopes exerted a negative effect on the subgroup of pTNM stage I carcinomas. These results indicate that TF and MUC1-TF immunoreactivity defines a 'high-risk' subgroup of stage I patients in gastric cancer.
汤姆森-弗里德赖希（TF）抗原是一种著名的人类泛癌抗原。它代表一种碳水化合物核心二糖（半乳糖β1-3N-乙酰半乳糖胺），主要与粘蛋白肽核心结合。其免疫反应性取决于糖基化的变化，这种变化会导致碳水化合物链长度缩短以及核心碳水化合物的暴露。在本研究中，我们应用两种单克隆抗体（MAb）对208例胃腺癌的免疫组化反应性进行了研究。包括特异性检测TF抗原的单克隆抗体（A78-G/A7）和单克隆抗体BW835。后者与MUC1肽核心的某种糖型反应，其特征是具有像TF这样的核心型聚糖。在68.8%的癌组织中检测到A78-G/A7表位，在57.7%的癌组织中检测到BW835表位。BW835免疫反应性与淋巴结转移的存在相关。根据劳伦分类，在管状/乳头状癌（世界卫生组织分类）和肠型癌中，A78-G/A7和BW835染色均明显更强。在对所有研究患者的单因素生存分析中，BW835免疫反应性是预后不良的一个标志物（p<0.05）。A78-G/A7和BW835表位的存在对pTNM I期癌亚组产生负面影响。这些结果表明，TF和MUC1-TF免疫反应性定义了胃癌I期患者中的一个“高危”亚组。
