Yang Fei, Li Zhe, Shao Jing-Bo, Gao Wei, Sun Zhi-Gang
Department of Pathology, Jinan Central Hospital Affiliated to Shandong University, Central Hospital Affiliated to Shandong First Medical University, Jinan, China.
Department of Medical Examination, Jinan Central Hospital Affiliated to Shandong University, Central Hospital Affiliated to Shandong First Medical University, Jinan, China.
Arch Med Sci. 2019 Oct 28;19(3):638-644. doi: 10.5114/aoms.2019.89219. eCollection 2023.
Lymph node metastasis (LNM) and lymph node micrometastasis (LNMM) are prognostic factors in esophageal squamous cell carcinoma (ESCC) patients. The purpose of this research is to investigate whether mucin 1 expression detected by immunohistochemistry in lymph nodes correlates with LNM, LNMM and prognosis in ESCC patients.
There were 92 ESCC patients enrolled in the research, and 1382 lymph nodes were obtained from these 92 patients. All lymph nodes were immunohistochemically analyzed using an anticytokeratin and mucin 1 antibody cocktail.
In the pN1-2 patients' group, 68 lymph nodes from 15 patients had tumor metastasis. All these 68 tumor metastatic lymph nodes were positive for mucin 1. Mucin 1 was detected in another 231 lymph nodes and among them, 3 (3/231 1.3%) lymph nodes from 2 (2/15 13.3%) patients were positive for mucin 1. In 77 pN0 patients, mucin 1 was detected in 1083 lymph nodes from the 77 patients; 17 (17/1083 1.6%) lymph nodes from 15 (15/77 19.5%) patients were positive for mucin 1. The 5-year survival rate was 39.1%, and it was significantly related to tumor invasion (pT, < 0.05), lymph node metastasis (pN, < 0.01), pTNM stage ( < 0.01) and mucin 1 expression ( < 0.01). Cox regression of multivariate analysis demonstrated that mucin 1 expression and pT were independent prognostic factors.
Mucin 1 expression was related to LNM and LNMM and poor prognosis in ESCC patients. Immunohistochemistry for mucin 1 can be applied for the detection of LNM and LNMM.
淋巴结转移（LNM）和淋巴结微转移（LNMM）是食管鳞状细胞癌（ESCC）患者的预后因素。本研究的目的是探讨通过免疫组织化学检测淋巴结中粘蛋白1的表达是否与ESCC患者的LNM、LNMM及预后相关。
本研究纳入92例ESCC患者，从这92例患者中获取了1382个淋巴结。所有淋巴结均使用抗细胞角蛋白和粘蛋白1抗体混合物进行免疫组织化学分析。
在pN1-2患者组中，15例患者的68个淋巴结发生了肿瘤转移。所有这68个肿瘤转移淋巴结的粘蛋白1均呈阳性。在另外231个淋巴结中检测到了粘蛋白1，其中来自2例（2/15，13.3%）患者的3个（3/231，1.3%）淋巴结粘蛋白1呈阳性。在77例pN0患者中，在77例患者的1083个淋巴结中检测到了粘蛋白1；来自15例（15/77，19.5%）患者的17个（17/1083，1.6%）淋巴结粘蛋白1呈阳性。5年生存率为39.1%，其与肿瘤浸润（pT，<0.05）、淋巴结转移（pN，<0.01）、pTNM分期（<0.01）及粘蛋白1表达（<0.01）显著相关。多因素分析的Cox回归表明，粘蛋白1表达和pT是独立的预后因素。
粘蛋白1表达与ESCC患者的LNM、LNMM及不良预后相关。粘蛋白1的免疫组织化学可用于检测LNM和LNMM。
