一种用于微循环视频记录数字化和分析的有效且经济的解决方案。

An effective and economical solution for digitizing and analyzing video recordings of the microcirculation.

作者信息

Norman K E

机构信息

Cardiovascular Research Group, Division of Clinical Sciences (Northern General Hospital), University of Sheffield, Sheffield, UK.

出版信息

Microcirculation. 2001 Aug;8(4):243-9. doi: 10.1038/sj/mn/7800082.

DOI:10.1038/sj/mn/7800082

PMID:11528532

Abstract

OBJECTIVE

To assemble an economical system for analysis of microcirculation movies.

METHODS

Images of cremaster microvessels were recorded onto sVHS video cassettes. These recordings were digitized onto a Macintosh computer using a MiroMotion DC30 plus video compression card and Adobe Premiere software, which permits video rate (25-30 fps) capture of full-sized (768 x 576 PAL, 640 x 480 NTSC) frames. Once captured, images were analyzed using NIH Image software.

RESULTS

Combination of the Macintosh computer, the MiroMotion card, and Adobe Premiere allowed capture of high-resolution images at video rate, with the only limitation to sequence length being available hard-drive space. Captured movies could be directly accessed using the freely available NIH Image software. Use of built-in analysis tools and custom-written macros greatly facilitated rapid, accurate, and reproducible analysis of parameters such as blood flow velocity, leukocyte rolling velocity, and firm adhesion.

CONCLUSIONS

Low-priced hardware and software aimed at the home-video enthusiast can be combined with free image-analysis software to provide a powerful image-analysis solution for study of the microcirculation.

摘要

目的

组装一个用于分析微循环影片的经济系统。

方法

将提睾肌微血管图像记录到超级家用录像系统（sVHS）录像带上。使用MiroMotion DC30 plus视频压缩卡和Adobe Premiere软件将这些录像数字化到苹果麦金塔电脑上，该软件允许以视频速率（25 - 30帧/秒）捕捉全尺寸（768×576 PAL、640×480 NTSC）帧。一旦捕捉到，就使用美国国立卫生研究院（NIH）的Image软件对图像进行分析。

结果

苹果麦金塔电脑、MiroMotion卡和Adobe Premiere的组合允许以视频速率捕捉高分辨率图像，序列长度的唯一限制是可用的硬盘空间。可以使用免费的NIH Image软件直接访问捕捉到的影片。使用内置分析工具和自定义编写的宏极大地促进了对诸如血流速度、白细胞滚动速度和牢固黏附等参数的快速、准确和可重复分析。