Norman K E
Cardiovascular Research Group, Division of Clinical Sciences (Northern General Hospital), University of Sheffield, Sheffield, UK.
Microcirculation. 2001 Aug;8(4):243-9. doi: 10.1038/sj/mn/7800082.
To assemble an economical system for analysis of microcirculation movies.
Images of cremaster microvessels were recorded onto sVHS video cassettes. These recordings were digitized onto a Macintosh computer using a MiroMotion DC30 plus video compression card and Adobe Premiere software, which permits video rate (25-30 fps) capture of full-sized (768 x 576 PAL, 640 x 480 NTSC) frames. Once captured, images were analyzed using NIH Image software.
Combination of the Macintosh computer, the MiroMotion card, and Adobe Premiere allowed capture of high-resolution images at video rate, with the only limitation to sequence length being available hard-drive space. Captured movies could be directly accessed using the freely available NIH Image software. Use of built-in analysis tools and custom-written macros greatly facilitated rapid, accurate, and reproducible analysis of parameters such as blood flow velocity, leukocyte rolling velocity, and firm adhesion.
Low-priced hardware and software aimed at the home-video enthusiast can be combined with free image-analysis software to provide a powerful image-analysis solution for study of the microcirculation.
组装一个用于分析微循环影片的经济系统。
将提睾肌微血管图像记录到超级家用录像系统（sVHS）录像带上。使用MiroMotion DC30 plus视频压缩卡和Adobe Premiere软件将这些录像数字化到苹果麦金塔电脑上，该软件允许以视频速率（25 - 30帧/秒）捕捉全尺寸（768×576 PAL、640×480 NTSC）帧。一旦捕捉到，就使用美国国立卫生研究院（NIH）的Image软件对图像进行分析。
苹果麦金塔电脑、MiroMotion卡和Adobe Premiere的组合允许以视频速率捕捉高分辨率图像，序列长度的唯一限制是可用的硬盘空间。可以使用免费的NIH Image软件直接访问捕捉到的影片。使用内置分析工具和自定义编写的宏极大地促进了对诸如血流速度、白细胞滚动速度和牢固黏附等参数的快速、准确和可重复分析。
面向家庭视频爱好者的低价硬件和软件可以与免费图像分析软件相结合，为微循环研究提供强大的图像分析解决方案。
