Kretzschmar E, Von Ahsen N, Trobisch H
Institute for Transfusion Medicine, Duisburg, Germany.
Br J Haematol. 2001 Aug;114(2):397-9. doi: 10.1046/j.1365-2141.2001.02951.x.
Genotyping of human platelet antigens (HPAs) can be useful for the diagnosis and therapy of alloimmune thrombocytopenic syndromes such as neonatal alloimmune thrombocytopenic purpura, post-transfusion purpura and refractoriness to platelet transfusion therapy. We developed a single-tube method for HPA-5 genotyping on the LightCycler that combines rapid-cycle polymerase chain reaction with allele-specific fluorescent probe melting for mutation detection. Our method is fast, robust and suitable for routine HPA-5 typing. This work extends recent studies on HPA-1 typing using the LightCycler.
人类血小板抗原（HPA）基因分型对于诊断和治疗同种免疫性血小板减少综合征（如新生儿同种免疫性血小板减少性紫癜、输血后紫癜以及血小板输注治疗无效）可能是有用的。我们在LightCycler上开发了一种用于HPA-5基因分型的单管方法，该方法将快速循环聚合酶链反应与等位基因特异性荧光探针熔解相结合以检测突变。我们的方法快速、可靠，适用于常规HPA-5分型。这项工作扩展了最近使用LightCycler对HPA-1分型的研究。
