Brès J-C, Mérieux Y, Dugas V, Broutin J, Vnuk E, Jaber M, Rigal D, Martin J-R, Souteyrand E, Cabrera M, Cloarec J-P
LEOM-UMR 5512, Ecole Centrale de Lyon, BP 163, 69131 Ecully Cedex, France.
Biomed Microdevices. 2005 Jun;7(2):137-41. doi: 10.1007/s10544-005-1593-0.
DNA microarrays are a powerful experimental tool for the detection of specific genomic sequences and are invaluable to a broad array of applications: clinical diagnosis, personalized medicine, drug research and development, gene therapy, food control technologies, and environmental sciences. Alloimmunization to human platelet antigens (HPAs) is commonly responsible for neonatal alloimmune thrombocytopenia, post-transfusional purpura and platelet transfusion refractoriness. Using DNA microarrays, we developed a diagnosis to type the biallelic HPA-1 platelet group. The region for the human genomic DNA sequence that contains the polymorphism responsible for HPA-1 alleles was amplified by polymerase chain reaction (PCR). The expected DNA fragments were hybridized on DNA microarrays, and the data were analyzed using specially developed software. Our initial results show that the two HPA-1 antigens polymorphisms containing a single base difference were detected using DNA microarrays.
DNA微阵列是用于检测特定基因组序列的强大实验工具，对广泛的应用领域都非常重要：临床诊断、个性化医疗、药物研发、基因治疗、食品控制技术和环境科学。对人类血小板抗原（HPA）的同种免疫通常是新生儿同种免疫性血小板减少症、输血后紫癜和血小板输注无效的原因。我们使用DNA微阵列开发了一种诊断方法，用于对双等位基因HPA-1血小板组进行分型。通过聚合酶链反应（PCR）扩增包含负责HPA-1等位基因多态性的人类基因组DNA序列区域。预期的DNA片段与DNA微阵列杂交，并使用专门开发的软件分析数据。我们的初步结果表明，使用DNA微阵列检测到了两个含有单个碱基差异的HPA-1抗原多态性。
