中性粒细胞通过组织蛋白酶G和弹性蛋白酶的作用促进血小板释放α-6-岩藻糖基转移酶。

Neutrophils promote the release of alpha-6-fucosyltransferase from blood platelets through the action of cathepsin G and elastase.

作者信息

Kamińska J, Musielak M, Nowicka A, Wozniewicz B, Kościelak J

机构信息

Department of Biochemistry, Institute of Hematology and Blood Transfusion, ul. Chocimska 5, 00-957 Warsaw, Poland.

出版信息

Biochimie. 2001 Aug;83(8):739-42. doi: 10.1016/s0300-9084(01)01306-2.

DOI:10.1016/s0300-9084(01)01306-2

PMID:11530205

Abstract

Human blood platelets release alpha-6-fucosyltransferase during coagulation of blood or after stimulation with thrombin or other agonists that cause platelet activation (Antoniewicz et al., FEBS Lett. 244 (1989) 388-390). However, in the absence of neutrophils the thrombin-stimulated platelets release only a small fraction of alpha-6-fucosyltransferase activity (Kościelak et al., Acta Biochim. Polon. 42 (1995) 35-40). We show that the effect of neutrophils is reproduced by cathepsin G or (less efficiently) by elastase, the two enzymes that are released by neutrophils during coagulation of blood. We have also localized alpha-6-fucosyltransferase to membrane and alpha-granule fractions of platelets that had been disrupted by nitrogen cavitation. It is concluded that thrombin-activated neutrophils release cathepsin G and elastase that promote degranulation of platelets and hence the secretion of alpha-6-fucosyltransferase.

摘要

人血小板在血液凝固过程中或经凝血酶或其他导致血小板活化的激动剂刺激后释放α-6-岩藻糖基转移酶（Antoniewicz等人，《欧洲生物化学学会联合会快报》244 (1989) 388 - 390）。然而，在没有中性粒细胞的情况下，凝血酶刺激的血小板仅释放一小部分α-6-岩藻糖基转移酶活性（Kościelak等人，《波兰生物化学学报》42 (1995) 35 - 40）。我们发现，组织蛋白酶G或（效率稍低的）弹性蛋白酶可重现中性粒细胞的作用，这两种酶是中性粒细胞在血液凝固过程中释放的。我们还将α-6-岩藻糖基转移酶定位到经氮空化破坏的血小板的膜和α-颗粒组分中。得出的结论是，凝血酶激活的中性粒细胞释放组织蛋白酶G和弹性蛋白酶，它们促进血小板脱颗粒，从而促进α-6-岩藻糖基转移酶的分泌。