[来自疑似疯草（歧茎棘豆（Pall）DC）的半乳甘露聚糖]

[Galactomannan from ambiguous Crazyweed (Oxytropis ambigua (Pall) DC)].

作者信息

Mestechkina N M, Lobanova I E, Anulov O V, Shcherbukhin V D

机构信息

Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr., 33, Moscow, 117071 Russia.

出版信息

Prikl Biokhim Mikrobiol. 2001 Jul-Aug;37(4):453-6.

PMID:11530670

Abstract

A galactomannan with a molecular weight of 735 kDa was first isolated and purified from seeds of ambiguous crazyweed Oxytropis ambigua (Pall) DC. (family Leguminosae) with a yield of 3.6%. Its aqueous solutions displayed an optical activity ([alpha]D = 73.32 degrees) and high viscosity ([eta] = 644 ml g-1). Chemical analysis and 13C-NMR spectroscopy revealed the presence in the heteropolysaccharide of D-mannopyranose and D-glucopyranose at a molar ratio of 1.39:1. The linear backbone of its macromolecule consists of 1.4-beta-D-mannopyranose residues. Single beta-D-galactose residues substitute 72% of mannoses to form branches.

摘要

首次从豆科植物变异黄芪（Oxytropis ambigua (Pall) DC.）种子中分离并纯化出一种分子量为735 kDa的半乳甘露聚糖，产率为3.6%。其水溶液具有旋光性（[α]D = 73.32°）和高粘度（[η] = 644 ml g-1）。化学分析和13C-NMR光谱表明，该杂多糖中D-甘露吡喃糖和D-葡糖吡喃糖的摩尔比为1.39:1。其大分子的线性主链由1,4-β-D-甘露吡喃糖残基组成。单个β-D-半乳糖残基取代72%的甘露糖形成分支。