Olennikov D N, Rokhin A V
Prikl Biokhim Mikrobiol. 2010 Jan-Feb;46(1):113-8.
By the hot water extraction method, galactomannan was extracted (4.5% yield of the seed mass) from the seeds of Chinese honey locust (Gleditsia sinensis Lam). It had a molecular weight of 1230 kDa, and its solutions had a high viscosity [eta] of 1064 ml/g and optical activity [alpha]D + 21.4 degrees. The polysaccharide consists of mannose and galactose residues in the molar ratio 2.69 : 1. In the galactomannan macromolecule the backbone is formed by 1,4-beta-D-mannopyranose residues, 37% of which are substituted by alpha-D-galactopyranose at C-6. By 13C-NMR-spectroscopy, fragments of differently galactose-substituted mannobiose units were found to be in the galactomannan being studied: Man-Man, (Gal)Man-Man, and Man-Man(Gal) in the ratio of 0.23 : 0.47 : 0.30.
采用热水提取法从皂荚（Gleditsia sinensis Lam）种子中提取出半乳甘露聚糖（占种子质量的产率为4.5%）。其分子量为1230 kDa，溶液具有1064 ml/g的高粘度[η]以及[α]D + 21.4°的旋光性。该多糖由甘露糖和半乳糖残基按摩尔比2.69 : 1组成。在半乳甘露聚糖大分子中，主链由1,4-β-D-甘露吡喃糖残基构成，其中37%在C-6位被α-D-半乳吡喃糖取代。通过13C-NMR光谱分析，在所研究的半乳甘露聚糖中发现了不同半乳糖取代的甘露二糖单元片段：Man-Man、(Gal)Man-Man和Man-Man(Gal)，其比例为0.23 : 0.47 : 0.30。
