Navarro F, Perez-Trallero E, Marimon J M, Aliaga R, Gomariz M, Mirelis B
Microbiology Department, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma 08025 Barcelona, Spain.
J Antimicrob Chemother. 2001 Sep;48(3):383-9. doi: 10.1093/jac/48.3.383.
CMY-2 plasmid-mediated AmpC beta-lactamase (CMY-2) was detected in 21 isolates from two hospitals located in different geographical regions of Spain between October 1999 and December 2000. The isolates comprised two Salmonella enterica serovars (Mikawasima and Montevideo), 16 Escherichia coli, one Klebsiella pneumoniae, one Klebsiella oxytoca and one Proteus mirabilis. In addition to the expected resistance to beta-lactams, including extended-spectrum cephalosporins and cefoxitin, all isolates showed a broad spectrum of associated resistance. All were resistant to sulfamethoxazole, chloramphenicol, tetracycline and streptomycin, and all but two were also resistant to gentamicin. Five isolates were studied in detail and all transferred CMY-2 and other resistance determinants by conjugation. Genomic DNA restriction pattern analysis of the E. coli isolates excluded the dissemination of a single clone. To the best of our knowledge this is the first time that CMY-2 has been detected in P. mirabilis, K. oxytoca and S. enterica serovars Mikawasima and Montevideo. It is also the first time that CMY-2 has been described in Spain.
1999年10月至2000年12月期间，在西班牙不同地理区域的两家医院的21株分离菌中检测到CMY-2质粒介导的AmpCβ-内酰胺酶（CMY-2）。这些分离菌包括两种肠炎沙门氏菌血清型（三河岛和蒙得维的亚）、16株大肠杆菌、1株肺炎克雷伯菌、1株产酸克雷伯菌和1株奇异变形杆菌。除了对β-内酰胺类药物（包括超广谱头孢菌素和头孢西丁）预期的耐药性外，所有分离菌均表现出广泛的相关耐药性。所有菌株均对磺胺甲恶唑、氯霉素、四环素和链霉素耐药，除两株外，所有菌株对庆大霉素也耐药。对5株分离菌进行了详细研究，所有菌株均通过接合转移CMY-2和其他耐药决定因素。对大肠杆菌分离菌的基因组DNA限制性图谱分析排除了单个克隆的传播。据我们所知，这是首次在奇异变形杆菌、产酸克雷伯菌以及肠炎沙门氏菌血清型三河岛和蒙得维的亚中检测到CMY-2。这也是CMY-2首次在西班牙被描述。
