Sunkara G, Mada S R, Vobalaboina V
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Kakatiya University, Warangal, India.
Pharmazie. 2001 Aug;56(8):640-2.
A reverse phase HPLC method to determine piperine, a pungent constituent of black pepper, in rabbit serum and various tissues of the rat was developed. A pharmacokinetic study was performed in rabbits and tissue distribution studies were carried out in rats. High reproducibility was achieved in quantitative analysis over the concentration range of 0.2-20 micrograms/ml serum. After bolus intravenous administration of piperine at a dose of 10 mg/kg, the serum concentration--time curve fitted the two-compartment open model. The tissue distribution pattern of piperine in rats also supports the two-compartment open model.
建立了一种反相高效液相色谱法，用于测定兔血清和大鼠各组织中胡椒碱（黑胡椒的一种辛辣成分）的含量。对家兔进行了药代动力学研究，并对大鼠进行了组织分布研究。在0.2 - 20微克/毫升血清浓度范围内进行定量分析，重现性良好。以10毫克/千克的剂量静脉推注胡椒碱后，血清浓度 - 时间曲线符合二室开放模型。胡椒碱在大鼠体内的组织分布模式也支持二室开放模型。
