Hao Xiuhua, Cheng Gang, Sun Jin, Zou Meijuan, Yu Jie, Zhang Shuang, Cui Fude
Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang 110016, China.
J Pharm Biomed Anal. 2005 Jun 15;38(2):360-3. doi: 10.1016/j.jpba.2005.01.004.
A validated HPLC method was developed for the quantification of scutellarin in rat plasma using a liquid-liquid extraction and an ultraviolet detection. Chromatographic separation of scutellarin in plasma was performed on a C18 column, with a mobile phase of acetonitrile-water (23:77, v/v), adjusted to pH 2.5 with 1M phosphoric acid, and rutin was used as an internal standard. The calibration curve was linear over the range 0.1-100 microg/ml in rat plasma. The average extraction recoveries were 85.9+/-8.9, 71.0+/-4.6, 72.7+/-1.2% (n=3) at concentrations of 0.1, 2, 100 microg/ml, respectively, and the within-day and between-day precisions were less than 15%. After intravenous administration to rats over the doses range of 10-40 mg/kg, the plasma concentration-time curve of scutellarin was best conformed to three-compartment open model. The AUC of scutellarin was proportional to dose, and the systemic clearance (Cl), elimination half-life (t1/2beta) and apparent volume of distribution (Vc) were not significantly different among the three doses, suggestive of the linear pharmacokinetics characteristic of scutellarin after intravenous administration.
建立了一种经过验证的高效液相色谱法，用于测定大鼠血浆中灯盏花素的含量，采用液-液萃取和紫外检测。血浆中灯盏花素的色谱分离在C18柱上进行，流动相为乙腈-水（23:77，v/v），用1M磷酸调节至pH 2.5，芦丁用作内标。校准曲线在大鼠血浆中0.1-100μg/ml范围内呈线性。在浓度分别为0.1、2、100μg/ml时，平均萃取回收率分别为85.9±8.9%、71.0±4.6%、72.7±1.2%（n=3），日内和日间精密度均小于15%。大鼠静脉注射剂量范围为10-40mg/kg后，灯盏花素的血浆浓度-时间曲线最符合三室开放模型。灯盏花素的AUC与剂量成正比，三种剂量下的全身清除率（Cl）、消除半衰期（t1/2β）和表观分布容积（Vc）无显著差异，提示灯盏花素静脉注射后具有线性药代动力学特征。
