Brouwer K C, Ndhlovu P, Munatsi A, Shiff C J
The W. Harry Feinstone Department of Molecular Microbiology & Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
J Parasitol. 2001 Aug;87(4):762-9. doi: 10.1645/0022-3395(2001)087[0762:GDOAPO]2.0.CO;2.
To characterize the extent of genetic diversity of Schistosoma haematobium within and among its definitive host (intra- and interhost parasite diversity), 133 individual isolates from 25 infected schoolchildren were compared using randomly amplified polymorphic DNA markers. With 4 primers, 53 unambiguous loci were identified, and of these, 22 were polymorphic. Mean heterozygosity in the population was 0.116 +/- 0.043. Analysis of molecular variance showed the majority of variance occurred within, rather than between, hosts. Frequencies of certain alleles segregated the parasite population into 13 distinct clusters of associated genotypes, with 4 of these first appearing 10 mo after the initial survey. Considering the level of diversity within this limited geographical area and the possibility of rapid turnover of genotypes, parasite variance may impact acquired immunity and clinical outcome of the infection.
为了描述埃及血吸虫在其终末宿主内和宿主间的遗传多样性程度（宿主内和宿主间寄生虫多样性），使用随机扩增多态性DNA标记对来自25名受感染学童的133个个体分离株进行了比较。使用4种引物，鉴定出53个明确的位点，其中22个是多态性的。群体中的平均杂合度为0.116±0.043。分子方差分析表明，大部分方差发生在宿主内而非宿主间。某些等位基因的频率将寄生虫群体分为13个不同的相关基因型簇，其中4个在初次调查后10个月首次出现。考虑到这一有限地理区域内的多样性水平以及基因型快速更替的可能性，寄生虫变异可能会影响获得性免疫和感染的临床结果。
