Brouwer Kimberly C, Ndhlovu Patricia D, Wagatsuma Yukiko, Munatsi Anderson, Shiff Clive J
The W. Harry Feinstone Department of Molecular Microbiology & Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
Am J Trop Med Hyg. 2003 Apr;68(4):456-62.
Disease outcome in persons infected with Schistosoma haematobium varies dramatically, ranging from mild symptoms to severe damage of the kidneys and/or bladder. We used ultrasonography to characterize the extent of urinary tract pathology of infected children in Zimbabwe, and random genetic markers to examine the relationship between genetic diversity of S. haematobium and clinical outcome. One hundred thirty-three parasite isolates from 12 students with mild lesions and 13 with severe lesions were compared. Using four randomly amplified polymorphic DNA (RAPD) markers, we scored parasite allelic frequencies at 53 loci. Although parasite heterogeneity did not differ, allelic frequencies at eight loci differed significantly between the mild and severe groups. Parasite isolates were analyzed further using a modified cluster analysis that segregated the population into 13 clusters of associated genotypes. Three clusters were significantly over-represented in children with severe lesions. Our findings, although preliminary, suggest that parasite genetic associations may be important in clinical outcome.
感染埃及血吸虫的人的疾病结局差异很大，从轻微症状到肾脏和/或膀胱的严重损伤不等。我们使用超声检查来描述津巴布韦感染儿童的尿路病理学程度，并使用随机遗传标记来研究埃及血吸虫的遗传多样性与临床结局之间的关系。比较了来自12名轻度病变学生和13名重度病变学生的133个寄生虫分离株。使用四个随机扩增多态性DNA（RAPD）标记，我们在53个位点对寄生虫等位基因频率进行了评分。尽管寄生虫的异质性没有差异，但轻度和重度组之间八个位点的等位基因频率存在显著差异。使用改良的聚类分析对寄生虫分离株进行进一步分析，该分析将群体分为13个相关基因型的聚类。三个聚类在重度病变儿童中显著过度代表。我们的发现虽然是初步的，但表明寄生虫的遗传关联可能对临床结局很重要。
