Dulay M T, Quirino J P, Bennett B D, Kato M, Zare R N
Department of Chemistry, Stanford University, California 94305-5080, USA.
Anal Chem. 2001 Aug 15;73(16):3921-6. doi: 10.1021/ac0100749.
A solution of methacryloxypropyltrimethoxysilane in the presence of an acid catalyst, water, toluene, and a photoinitiator was irradiated at 365 nm for 5 min in a 75-microm i.d. capillary to prepare a porous monolithic sol-gel column by a one-step, in situ, process. The photopolymerized sol-gel (PSG) column shows reversed-phase behavior. Using this column, a variety of low-molecular-weight neutral compounds, including polycyclic aromatic hydrocarbons, alkyl benzenes, alkyl phenyl ketones, and steroids are separated from mixtures. Various different operational parameters, such as buffer composition, field strength, and column temperature, were varied to assess their influence on column performance. Use of PSG as a stationary phase for a pressure-driven separation is also demonstrated.
在酸催化剂、水、甲苯和光引发剂存在的情况下，将甲基丙烯酰氧基丙基三甲氧基硅烷溶液在内径为75微米的毛细管中于365纳米下照射5分钟，通过一步原位法制备多孔整体式溶胶-凝胶柱。光聚合溶胶-凝胶（PSG）柱表现出反相行为。使用该柱可从混合物中分离出多种低分子量中性化合物，包括多环芳烃、烷基苯、烷基苯基酮和类固醇。改变各种不同的操作参数，如缓冲液组成、场强和柱温，以评估它们对柱性能的影响。还证明了使用PSG作为压力驱动分离的固定相。
