Levy Miriam H, Goswami Shubhodeep, Plawsky Joel, Cramer Steven M
Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 Eighth Street, Troy, NY 12180.
Chromatographia. 2013 Aug;76(15):993-1002. doi: 10.1007/s10337-013-2493-8. Epub 2013 Jun 26.
Although polydimethylsiloxane (PDMS) microfluidic chips provide an alternative to more expensive microfabricated glass chips, formation of monolithic stationary phases in PDMS is not a trivial task. Photopolymerized silica sol-gel monoliths were fabricated in PDMS based microfluidic devices using 3-trimethoxysilylpropylmethacrylate (MPTMOS) and glycidyloxypropyltrimethoxysilane (GPTMOS). The monolith formation was optimized by identifying a suitable porogen, controlling monomer concentration, functional additives, salts, porogen, wall attachment methods, and rinsing procedures. The resulting monoliths were evaluated using scanning electron microscopy, image analysis, differential scanning calorimetry, and separation performance. Monoliths functionalized with boronic acid ligands were used for the separation of cis-diol containing compounds both in batch mode and in the microfluidic chip.
尽管聚二甲基硅氧烷（PDMS）微流控芯片为更昂贵的微加工玻璃芯片提供了一种替代方案，但在PDMS中形成整体固定相并非易事。使用甲基丙烯酸3-三甲氧基硅丙酯（MPTMOS）和环氧丙氧基丙基三甲氧基硅烷（GPTMOS），在基于PDMS的微流控装置中制备了光聚合二氧化硅溶胶-凝胶整体柱。通过确定合适的致孔剂、控制单体浓度、功能添加剂、盐、致孔剂、壁附着方法和冲洗程序，对整体柱的形成进行了优化。使用扫描电子显微镜、图像分析、差示扫描量热法和分离性能对所得整体柱进行了评估。用硼酸配体功能化的整体柱用于在分批模式和微流控芯片中分离含顺式二醇的化合物。
