Kang J W, Jeong Y J, Kwon A R, Yun H J, Kim D H, Choi E C
College of Pharmacy, Seoul National University, Korea.
Arch Pharm Res. 2001 Aug;24(4):316-22. doi: 10.1007/BF02975099.
Arylsulfate sulfotransferase (ASST) transfers a sulfate group from a phenolic sulfate ester to a phenolic acceptor substrate. In the present study, the gene encoding ASST was cloned from a genomic library copy of Citrobacter freundii, subcloned into the vector pGEM3Zf(-) and sequenced. Sequencing revealed two contiguous open reading frames (ORF1 and ORF2) on the same strand and based on amino acid sequence homology, they were designated as astA and dsbA, respectively. The amino acid sequence of astA deduced from C. freundii was highly similar to that of the Salmonella typhimurium, Enterobacter amnigenus, Klebsiella, Pseudomonas putida, and Campylobacter jejuni, encoded by the astA genes. However, the ASST activity assay revealed different acceptor specificities. Using p-nitrophenyl sulfate (PNS) as a donor substrate, alpha-naphthol was found to be the best acceptor substrate, followed by phenol, resorcinol, p-acetaminophen, tyramine and tyrosine.
芳基硫酸酯磺基转移酶（ASST）将硫酸基团从酚硫酸酯转移至酚类受体底物。在本研究中，编码ASST的基因从弗氏柠檬酸杆菌的基因组文库拷贝中克隆出来，亚克隆至载体pGEM3Zf(-) 并进行测序。测序揭示了同一链上两个相邻的开放阅读框（ORF1和ORF2），基于氨基酸序列同源性，它们分别被命名为astA和dsbA。从弗氏柠檬酸杆菌推导的astA氨基酸序列与鼠伤寒沙门氏菌、产氨肠杆菌、克雷伯氏菌、恶臭假单胞菌和空肠弯曲菌的astA基因编码序列高度相似。然而，ASST活性测定显示出不同的受体特异性。以对硝基苯硫酸酯（PNS）作为供体底物时，发现α-萘酚是最佳受体底物，其次是苯酚、间苯二酚、对乙酰氨基酚、酪胺和酪氨酸。
