Li H, Hickman C J, Helfand R F, Keyserling H, Anderson L J, Bellini W J
Respiratory and Enteric Viruses Branch, Measles Section MS. D11, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.
Vaccine. 2001 Sep 14;19(32):4896-900. doi: 10.1016/s0264-410x(01)00227-4.
To better characterize the cytokine response to measles virus vaccine, we examined the levels of IL-2, IL-4, IL-5, IL-10, IL-12 and gamma-interferon (gamma-IFN) in measles virus-stimulated peripheral blood mononuclear cells from 18 donors before and 2 weeks after vaccination. Donors were grouped as seropositive or seronegative on the basis of measles-specific IgM antibody present at 2 weeks postvaccination. After vaccination, similar levels of upregulation of IL-2 and gamma-IFN mRNA were observed in the two groups. The majority of donors in both groups did not exhibit an increase in measles specific IL-4 or IL-10 mRNA after vaccination. IL-12 mRNA was not induced by measles virus in any of the donors. A statistically significant upregulation of IL-5 mRNA was observed among seropositive (9/13) compared with seronegative (1/5) donors after vaccination (P=0.09, one tailed Fisher's test). The observed measles specific induction of IL-5 mRNA is suggestive of a possible association between IL-5 production and an antibody response to measles virus.
为了更好地表征对麻疹病毒疫苗的细胞因子反应，我们检测了18名供体接种疫苗前和接种后2周时，麻疹病毒刺激的外周血单核细胞中白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-5（IL-5）、白细胞介素-10（IL-10）、白细胞介素-12（IL-12）和γ-干扰素（γ-IFN）的水平。根据接种疫苗后2周时存在的麻疹特异性IgM抗体，将供体分为血清阳性或血清阴性。接种疫苗后，两组中IL-2和γ-IFN mRNA的上调水平相似。两组中的大多数供体在接种疫苗后麻疹特异性IL-4或IL-10 mRNA未出现增加。在任何供体中，麻疹病毒均未诱导IL-12 mRNA。接种疫苗后，血清阳性供体（9/13）中IL-5 mRNA的上调与血清阴性供体（1/5）相比具有统计学意义（P = 0.09，单尾Fisher检验）。观察到的麻疹特异性IL-5 mRNA诱导提示IL-5产生与对麻疹病毒的抗体反应之间可能存在关联。
