Kutil B L, Williams C G
Graduate Genetics Program, Texas A&M University, College Station, Texas 77843-2135, USA.
J Hered. 2001 Jul-Aug;92(4):327-32. doi: 10.1093/jhered/92.4.327.
Vascular plant species have shown a low level of microsatellite conservation compared to many animal species. Finding trans-specific microsatellites for plants may be improved by using a priori knowledge of genome organization. Fifteen triplet-repeat microsatellites from hard pine (Pinus taeda L.) were tested for trans-specific amplification across seven hard pines (P. palustris Mill., P. echinata Mill., P. radiata D. Don., P. patula Schiede et Deppe, P. halepensis Mill., P. kesiya Royle), a soft pine (P. strobus L.), and Picea rubens Sargent. Seven of 15 microsatellites had trans-specific amplification in both hard and soft pine subgenera. Two P. taeda microsatellites had conserved flanking regions and repeat motifs in all seven hard pines, soft pine P. strobus, and P. rubens. Perfect triplet-repeat P. taeda microsatellites appear to be better candidates for trans-specific polymorphism than compound microsatellites. Not all perfect triplet-repeat microsatellites were conserved, but all conserved microsatellites had perfect repeat motifs. Persistent microsatellites PtTX2123 and PtTX3020 had highly conserved flanking regions and a conserved repeat motif composition with variable repeat unit numbers. Using trinucleotide microsatellites improved trans-specific microsatellite recovery among hard and soft pine species.
与许多动物物种相比，维管植物物种的微卫星保守程度较低。利用基因组组织的先验知识，可能会提高寻找植物跨物种微卫星的效率。对来自火炬松（Pinus taeda L.）的15个三联体重复微卫星进行了测试，以检测其在7种硬松（湿地松P. palustris Mill.、短叶松P. echinata Mill.、辐射松P. radiata D. Don.、展叶松P. patula Schiede et Deppe、海岸松P. halepensis Mill.、思茅松P. kesiya Royle）、1种软松（北美乔松P. strobus L.）和红云杉（Picea rubens Sargent）中的跨物种扩增情况。15个微卫星中有7个在硬松亚属和软松亚属中都有跨物种扩增。2个火炬松微卫星在所有7种硬松、软松北美乔松和红云杉中都有保守的侧翼区域和重复基序。完美的三联体重复火炬松微卫星似乎比复合微卫星更适合作为跨物种多态性的候选者。并非所有完美的三联体重复微卫星都是保守的，但所有保守的微卫星都有完美的重复基序。持久性微卫星PtTX2123和PtTX3020具有高度保守的侧翼区域和保守的重复基序组成，只是重复单元数量可变。使用三核苷酸微卫星提高了硬松和软松物种间跨物种微卫星的回收率。
