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从无患子科植物中开发的简单序列重复(SSR)标记在无患子科植物间的可转移性

Transferability of Simple Sequence Repeat (SSR) Markers Developed in to (Sapindaceae).

作者信息

Ekué Marius R M, Gailing Oliver, Finkeldey Reiner

出版信息

Plant Mol Biol Report. 2009;27(4):570-574. doi: 10.1007/s11105-009-0115-2. Epub 2009 Jun 10.

Abstract

Ackee (, Sapindaceae) is a multipurpose fruit tree species of high economic importance, native to the Guinean forests of West Africa, and belongs to the same family as that of lychee (). In this study, a set of 12 primer pairs for simple sequence repeats (SSRs) previously developed for lychee has been evaluated for polymorphism in 16 ackee trees from different populations. Seven primer pairs have been found to be transferable, and four have revealed polymorphisms. However, the average number of alleles per locus has dropped from 4.9 for lychee to 3.7 for ackee. Characterization of the four polymorphic markers in 279 individuals belonging to14 different ackee populations from Benin has revealed that the numbers of alleles per locus range from two to 14 with a mean number of 5.8. The observed and expected heterozygosities range between 0.020 to 0.359 and 0.020 to 0.396, respectively.

摘要

西非荔枝(无患子科)是一种具有高度经济重要性的多用途果树,原产于西非几内亚森林,与荔枝同属一科。在本研究中,对先前为荔枝开发的一组12对简单序列重复(SSR)引物进行了评估,以检测来自不同种群的16棵西非荔枝树的多态性。已发现7对引物可转移,其中4对显示出多态性。然而,每个位点的平均等位基因数已从荔枝的4.9个降至西非荔枝的3.7个。对来自贝宁14个不同西非荔枝种群的279个个体中的4个多态性标记进行表征,结果显示每个位点的等位基因数在2至14个之间,平均为5.8个。观察到的杂合度和预期杂合度分别在0.020至0.359和0.020至0.396之间。

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