Saito Mitsumasa, Ohga Shouichi, Endoh Miyoko, Nakayama Hiroaki, Mizunoe Yoshimitsu, Hara Toshiro, Yoshida Shin-Ichi
Departments of Bacteriology1 and Pediatrics2, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Department of Microbiology, Tokyo Metropolitan Research and Laboratory of Public Health, Tokyo 169-0073, Japan3.
Microbiology (Reading). 2001 Sep;147(Pt 9):2469-2477. doi: 10.1099/00221287-147-9-2469.
The production of hydrogen peroxide (H(2)O(2)) and related phenotypes were studied with Streptococcus pyogenes strains isolated from cases of pharyngitis or severe group A streptococcal infections. Of the 46 strains examined (34 from severe infections and 12 from pharyngitis cases), 25 strains accumulated H(2)O(2) in the culture medium when grown under glucose-limited, aerobic conditions, whereas the rest of the strains did not. There was no correlation between these traits and the type of disease from which each strain had been isolated. The H(2)O(2)-nonproducing strains tested in this study belonged to T type 3 or T type 12. The accumulation of H(2)O(2) started when the culture reached the late exponential phase. A rapid loss of cell viability accompanied H(2)O(2) accumulation but was completely prevented by the addition of a catalase, indicating that the lethality was actually caused by H(2)O(2). Cells of H(2)O(2)-nonproducing strains were resistant to killing by phagocytes from patients with chronic granulomatous disease (CGD), whereas those of H(2)O(2)-producing strains were subject to killing. Subcutaneous inoculation of 10(5) c.f.u. H(2)O(2)-nonproducing S. pyogenes strains into the hind footpads of CGD mice provoked more prominent swelling of the footpad than did H(2)O(2)-producing strains. The mortality rate in the CGD mice infected with the H(2)O(2)-nonproducing strains was higher than that produced by the H(2)O(2)-producing strains. It is suggested that H(2)O(2)-nonproducing S. pyogenes strains are prevalent in humans and that they may be a potential threat to the health of CGD patients.
我们对从咽炎病例或严重的A组链球菌感染病例中分离出的化脓性链球菌菌株的过氧化氢（H₂O₂）产生情况及相关表型进行了研究。在所检测的46株菌株中（34株来自严重感染病例，12株来自咽炎病例），25株在葡萄糖受限的有氧条件下培养时会在培养基中积累H₂O₂，而其余菌株则不会。这些特性与各菌株所分离出的疾病类型之间没有相关性。本研究中检测的不产生H₂O₂的菌株属于T3型或T12型。H₂O₂的积累在培养进入指数后期时开始。细胞活力的快速丧失伴随着H₂O₂的积累，但通过添加过氧化氢酶可完全阻止这种情况，这表明致死性实际上是由H₂O₂引起的。不产生H₂O₂的菌株细胞对慢性肉芽肿病（CGD）患者的吞噬细胞杀伤具有抗性，而产生H₂O₂的菌株细胞则会被杀伤。将10⁵cfu不产生H₂O₂的化脓性链球菌菌株皮下接种到CGD小鼠的后足垫中，比接种产生H₂O₂的菌株引发的足垫肿胀更明显。感染不产生H₂O₂菌株的CGD小鼠的死亡率高于感染产生H₂O₂菌株的小鼠。这表明不产生H₂O₂的化脓性链球菌菌株在人类中普遍存在，并且它们可能对CGD患者的健康构成潜在威胁。
