单次抗原吸入诱导的肺迟发型超敏反应传出相期间支气管肺泡灌洗液中的CD4/CD8淋巴细胞

CD4/CD8 lymphocytes in BALF during the efferent phase of lung delayed-type hypersensitivity reaction induced by single antigen inhalation.

作者信息

Grubek-Jaworska H, Hoser G, Droszcz P, Chazan R

机构信息

Department and Clinic of Internal Medicine, Pulmonology and Allergology, Medical University, Warsaw, Poland.

出版信息

Med Sci Monit. 2001 Sep-Oct;7(5):878-83.

PMID:11535927

Abstract

BACKGROUND

The precise mechanisms involved in the pathogenesis of hypersensitivity pneumonitis (HP) have not been identified. HP is characterized by inflammatory lymphocytic alveolitis and a remarkable increase in T-lymphocytes detected in bronchoalveolar lavage fluid (BALF). It is suggested that both CD4+ and CD8+ T cells may contribute to the pathogenesis of HP. Experiments on animal models suggest that cell mediated immunity (CMI) is more important for the pathogenesis of HP than complex-mediated immunity, but the relationship between the subsets of BALF lymphocytes and humoral or cell-mediated allergic reactions is still not clear. The aim of our study was distinguish CD4+ and CD8+ T cells in BALF lymphocytes during a delayed-type hypersensitivity (DTH) reaction in the lung.

MATERIAL AND METHODS

The experiment was performed on guinea pigs sensitized with BCG vaccine and subjected to a single inhalation of tubercle bacilli antigens (tuberculin). 24 hours after tuberculin provocation (at the time of maximum lymphocyte infiltration), bronchoalveolar lavage was performed on both sensitized and non-sensitized (control) animals. The total cell count was estimated, and a differential microscopical examination of BAL-fluid cells was performed, along with the phenotyping of BALF lymphocytes (by flow cytometry).

RESULTS

In the BALF of the sensitized animals, as compared to the controls, there was a statistically significant increase in the percentage and absolute count of T-lymphocytes, CD4+ and CD8+. The CD4 / CD8 ratio in both groups did not differ significantly and was individually variable (2.94I0.72 SEM in the experimental group, vs 4.41I1.29 SEM in the control group).

CONCLUSIONS

Both CD4+ and CD8+ lymphocytes (with some predominance of helper cells) participate in the efferent phase of the delayed type hypersensitivity reaction in the lung induced by antigen inhalation.

摘要

背景

过敏性肺炎（HP）发病机制中的确切机制尚未明确。HP的特征是炎症性淋巴细胞肺泡炎以及在支气管肺泡灌洗（BALF）中检测到T淋巴细胞显著增加。有研究表明，CD4⁺和CD8⁺ T细胞可能都参与了HP的发病机制。动物模型实验表明，细胞介导免疫（CMI）在HP发病机制中比补体介导免疫更重要，但BALF淋巴细胞亚群与体液或细胞介导的过敏反应之间的关系仍不明确。我们研究的目的是区分肺部迟发型超敏反应（DTH）期间BALF淋巴细胞中的CD4⁺和CD8⁺ T细胞。