Boras Z, Juretić A, Gagro A, Pavelić Lj
Jordanovac University Hospital for Lung Diseases, Zagreb, Croatia.
Scand J Immunol. 2007 Mar;65(3):257-64. doi: 10.1111/j.1365-3083.2006.01890.x.
The possible immunological relationship between the pattern of Th1/Th2 cytokine production and tuberculin reactivity was assessed in patients with active Mycobacterium tuberculosis infection. The production of the intracellular cytokines interferon (IFN)-gamma and interleukin-4 (IL-4) was measured in CD4(+) and CD8(+) T cells obtained from peripheral blood and bronchoalveolar lavage fluid (BALF) of 20 tuberculin skin-positive patients and compared with the findings recorded in nine tuberculin skin-negative patients with active pulmonary tuberculosis. Upon stimulation with phorbol 12-myristate acetate/ionomycin for 6 h, tuberculin-negative patients had a significantly higher proportion of IFN-gamma-producing CD4(+) T lymphocytes in BALF than in peripheral blood, while both CD4(+) and CD8(+) T-lymphocyte subsets in BALF of tuberculin-positive patients secreted more IFN-gamma than their peripheral blood counterparts. Tuberculin-negative patients had a significantly higher proportion of IFN-gamma-producing CD4(+) T lymphocytes in peripheral blood than tuberculin-positive patients. There was no significant difference in the production of IFN-gamma by BALF CD4(+) T lymphocytes, or by either peripheral blood or BALF CD8(+) T lymphocytes. In two tuberculin-negative patients, peripheral blood CD4(+) T lymphocytes produced IL-4. Study results suggested a higher immune activity in the blood of tuberculin-negative patients, with an increased lymphocyte activity in BALF versus peripheral blood in both patient groups.
在活动性结核分枝杆菌感染患者中评估了Th1/Th2细胞因子产生模式与结核菌素反应性之间可能存在的免疫关系。对20例结核菌素皮肤试验阳性患者外周血及支气管肺泡灌洗液（BALF）中分离出的CD4(+)和CD8(+) T细胞内细胞因子干扰素（IFN）-γ和白细胞介素-4（IL-4）的产生情况进行了检测，并与9例活动性肺结核结核菌素皮肤试验阴性患者的检测结果进行比较。在用佛波酯12-肉豆蔻酸酯/离子霉素刺激6小时后，结核菌素阴性患者BALF中产生IFN-γ的CD4(+) T淋巴细胞比例显著高于外周血，而结核菌素阳性患者BALF中的CD4(+)和CD8(+) T淋巴细胞亚群分泌的IFN-γ均多于其外周血对应细胞。结核菌素阴性患者外周血中产生IFN-γ的CD4(+) T淋巴细胞比例显著高于结核菌素阳性患者。BALF中CD4(+) T淋巴细胞、外周血或BALF中CD8(+) T淋巴细胞产生IFN-γ的情况无显著差异。在2例结核菌素阴性患者中，外周血CD4(+) T淋巴细胞产生IL-4。研究结果表明，结核菌素阴性患者血液中的免疫活性较高，两组患者BALF中的淋巴细胞活性均高于外周血。
