Palmer J M, Warpeha K M, Briggs W R
Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305, USA.
Plant Physiol. 1996 Apr;110(4):1323-8. doi: 10.1104/pp.110.4.1323.
The photoreceptor that mediates blue-light-induced phototropism in dark-grown seedlings of higher plants has not been identified, although the carotenoid zeaxanthin has recently been proposed as the putative chromophore. In the experiments described in this paper, we analyzed phototropism and a blue-light-induced protein phosphorylation that has been genetically and physiologically implicated in phototropism in wild-type maize (Zea mays L.) seedlings and compared the results with those from seedlings that are either carotenoid deficient through a genetic lesion or have been chemically treated to block carotenoid biosynthesis. The blue-light-dependent phototropism and phosphorylation responses of seedlings deficient in carotenoids are the same as those of seedlings containing normal levels of carotenoids. These results and those in the literature make it unlikely that zeaxanthin or any other carotenoid is the chromophore of the blue-light photoreceptor for phototropism or the blue-light-induced phosphorylation related to phototropism.
尽管最近有人提出类胡萝卜素玉米黄质可能是发色团，但介导高等植物黑暗中生长的幼苗蓝光诱导向光性的光感受器尚未确定。在本文所述的实验中，我们分析了野生型玉米（Zea mays L.）幼苗的向光性以及一种在遗传和生理上与向光性有关的蓝光诱导蛋白磷酸化，并将结果与通过遗传损伤缺乏类胡萝卜素或经过化学处理以阻断类胡萝卜素生物合成的幼苗的结果进行了比较。缺乏类胡萝卜素的幼苗的蓝光依赖性向光性和磷酸化反应与含有正常水平类胡萝卜素的幼苗相同。这些结果以及文献中的结果表明，玉米黄质或任何其他类胡萝卜素不太可能是介导向光性的蓝光光感受器的发色团，也不太可能是与向光性相关的蓝光诱导磷酸化的发色团。
