Lin C, Robertson D E, Ahmad M, Raibekas A A, Jorns M S, Dutton P L, Cashmore A R
Department of Biology, University of Pennsylvania, Philadelphia, 19104, USA.
Science. 1995 Aug 18;269(5226):968-70. doi: 10.1126/science.7638620.
The arabidopsis thaliana HY4 gene encodes CRY1, a 75-kilodalton flavoprotein mediating blue light-dependent regulation of seedling development. CRY1 is demonstrated here to noncovalently bind stoichiometric amounts of flavin adenine dinucleotide (FAD). The redox properties of FAD bound by CRY1 include an unexpected stability of the neutral radical flavosemiquinone (FADH.). The absorption properties of this flavosemiquinone provide a likely explanation for the additional sensitivity exhibited by CRY1-mediated responses in the green region of the visible spectrum. Despite the sequence homology to microbial DNA photolyases, CRY1 was found to have no detectable photolyase activity.
拟南芥HY4基因编码CRY1，一种75千道尔顿的黄素蛋白，介导蓝光依赖的幼苗发育调控。本文证明CRY1以非共价方式结合化学计量的黄素腺嘌呤二核苷酸（FAD）。CRY1结合的FAD的氧化还原特性包括中性自由基黄素半醌（FADH.）的意外稳定性。这种黄素半醌的吸收特性可能解释了CRY1介导的反应在可见光谱绿色区域表现出的额外敏感性。尽管与微生物DNA光解酶有序列同源性，但发现CRY1没有可检测到的光解酶活性。
