微重力对海胆受精和发育的影响。

Microgravity effects on sea urchin fertilization and development.

作者信息

Steffen S, Fiser R, Simerly C, Schatten H, Schatten G

机构信息

Department of Zoology and Integrated Microscopy Resource, University of Wisconsin, Madison 53706, USA.

出版信息

Adv Space Res. 1992;12(1):167-73. doi: 10.1016/0273-1177(92)90280-b.

DOI:10.1016/0273-1177(92)90280-b

PMID:11536954

Abstract

Gravity has been a pervasive influence on all living systems and there is convincing evidence to suggest that it alters fertilization and embryogenesis in several developmental systems. Notwithstanding the global importance of gravity on development, it has only been recently possible to begin to design experiments which might directly investigate the specific effects of this vector. The goal of this research program is to explore and understand the effects of gravity on fertilization and early development using sea urchins as a model system. Sea urchin development has several advantages for this project including the feasibility of maintaining and manipulating these cells during spaceflight, the high percentage of normal fertilization and early development, and the abundant knowledge about molecular, biochemical, and cellular events during embryogenesis which permits detailed insights into the mechanism by which gravity might interfere with development. Furthermore, skeletal calcium is deposited into the embryonic spicules within a day of fertilization permitting studies of the effects of gravity on bone calcium deposition.

摘要

重力对所有生物系统都有着普遍影响，有确凿证据表明它会改变多个发育系统中的受精和胚胎发育过程。尽管重力对发育具有全球范围内的重要性，但直到最近才有可能开始设计实验，直接探究这个矢量的具体影响。本研究项目的目标是利用海胆作为模型系统，探索并理解重力对受精和早期发育的影响。海胆发育对于这个项目具有几个优势，包括在太空飞行期间维持和操控这些细胞的可行性、正常受精和早期发育的高比例，以及关于胚胎发生过程中分子、生化和细胞事件的丰富知识，这使得能够深入洞察重力可能干扰发育的机制。此外，受精后一天内骨骼钙就会沉积到胚胎骨针中，这便于研究重力对骨钙沉积的影响。