利用水生研究设施和受精注射器装置研究海胆在太空的发育情况。

Utilization of the aquatic research facility and fertilization syringe unit to study sea urchin development in space.

作者信息

Schatten H, Chakrabarti A, Levine H G, Anderson K

机构信息

Department of Veterinary Pathobiology, University of Missouri-Columbia 65211, USA.

出版信息

J Gravit Physiol. 1999 Oct;6(2):43-53.

PMID:11543085

Abstract

Methods were developed for the investigation of the effects of microgravity on early development in sea urchins within the Canadian Space Agency's Aquatic Research Facility (ARF). The ARF payload provided light, temperature control, automated fixation capability, and a 1 G on-orbit centrifuge control. Eggs and embryos of either the sea urchin species Lytechinus pictus or Strongylocentrotus purpuratus were loaded into Standard Container Assemblies (SCAs) which comprised the experimental aquaria (33 mL volume) contained within the ARF. A newly developed Fertilization Syringe Unit (FSU) was used to achieve "in-flight" fertilization capability. Fixative solutions were preloaded into fixation blocks maintained adjacent to the SCAs and injected at pre-selected time points, resulting in final (diluted) concentrations of either 0.5% or 2% glutaraldehyde (depending upon embryonic stage). Light, scanning, and transmission electron microscopy determined that all desired embryonic and cell division stages (16-cell stage, blastula, gastrula, and pluteus) were preserved using the experimental protocols and fixation capability provided by the ARF/FSU system.

摘要

在加拿大航天局的水生研究设施（ARF）内，开发了用于研究微重力对海胆早期发育影响的方法。ARF有效载荷提供光照、温度控制、自动固定能力以及在轨1G离心控制。将紫海胆或紫球海胆的卵和胚胎装入标准容器组件（SCA）中，这些组件构成了ARF内的实验水族箱（体积为33毫升）。使用新开发的受精注射器单元（FSU）实现“飞行中”受精能力。固定剂溶液预先装入与SCA相邻的固定块中，并在预先选定的时间点注入，最终（稀释）浓度为0.5%或2%戊二醛（取决于胚胎阶段）。光学显微镜、扫描电子显微镜和透射电子显微镜确定，使用ARF/FSU系统提供的实验方案和固定能力，可以保存所有所需的胚胎和细胞分裂阶段（16细胞期、囊胚、原肠胚和幼体）。