重离子对小鼠C3H 10T1/2细胞和叙利亚仓鼠胚胎细胞的致瘤转化

Neoplastic transformation of mouse C3H 10T1/2 and Syrian hamster embryo cells by heavy ions.

作者信息

Hieber L, Ponsel G, Trutschler K, Fenn S, Kellerer A M

机构信息

Institut fur Medizinische Strahlenkunde der Universitat Wurzburg, F.R.G.

出版信息

Adv Space Res. 1989;9(10):141-9. doi: 10.1016/0273-1177(89)90432-8.

DOI:10.1016/0273-1177(89)90432-8

PMID:11537286

Abstract

C3H 10T1/2 mouse-embryo fibroblasts were used for transformation experiments to study the effectiveness of various heavy ions with energies up to 20 MeV/u and LET values from 170 to 16,000 keV/micrometers. The transformation frequency per unit absorbed dose decreased with increasing ionization density; at the highest values of LET we found a decrease even of the transformation efficiency per unit fluence. Uranium ions at energies of 5, 9, and 16.3 MeV/u did not induce any transformation. In additional studies primary Syrian hamster embryo cells (SHE) were exposed to heavy ions in order to characterize cytological and molecular changes which may be correlated with neoplastic transformation. Growth behaviour, chromosomal status, tumorigenicity in nude mice, and expression of oncogenes of transformed cell lines were examined

摘要

使用C3H 10T1/2小鼠胚胎成纤维细胞进行转化实验，以研究能量高达20 MeV/u且线性能量传递（LET）值在170至16,000 keV/微米之间的各种重离子的有效性。单位吸收剂量的转化频率随电离密度的增加而降低；在LET的最高值时，我们发现单位注量的转化效率甚至也有所下降。能量为5、9和16.3 MeV/u的铀离子未诱导任何转化。在另外的研究中，将原代叙利亚仓鼠胚胎细胞（SHE）暴露于重离子，以表征可能与肿瘤转化相关的细胞学和分子变化。检查了转化细胞系的生长行为、染色体状态、裸鼠致瘤性和癌基因表达。