Church D L, Galston A W
Department of Biology, Yale University, New Haven, Connecticut 06511, USA.
Plant Physiol. 1988;88(1):92-6. doi: 10.1104/pp.88.1.92.
Mechanically isolated mesophyll cells of Zinnia elegans L. cv Envy differentiate to tracheary elements when cultured in inductive medium containing 0.5 micromolar alpha-naphthaleneacetic acid and 0.5 micromolar benzyladenine. The cells do not differentiate when cultured in medium in which the concentration of auxin and/or cytokinin has been reduced to 0.005 micromolar. Cells require an initial 24-hour exposure to inductive cytokinin and 56-hour exposure to inductive auxin for differentiation at 72 hours of culture. Freshly isolated Zinnia cells can be maintained in medium having low concentrations of both auxin and cytokinin for only 1 day without significant loss of potential to differentiate upon transfer to inductive medium. Initial culture for up to 2 days in medium having high auxin and low cytokinin, or low auxin and high cytokinin, allows full differentiation on the third day after transfer to inductive medium and potentiates the early differentiation of some cells.
百日草（Zinnia elegans L. cv Envy）的机械分离叶肉细胞在含有0.5微摩尔α-萘乙酸和0.5微摩尔苄基腺嘌呤的诱导培养基中培养时会分化为管状分子。当在生长素和/或细胞分裂素浓度已降至0.005微摩尔的培养基中培养时，细胞不会分化。细胞需要最初24小时暴露于诱导性细胞分裂素以及56小时暴露于诱导性生长素才能在培养72小时时进行分化。刚分离的百日草细胞在生长素和细胞分裂素浓度均较低的培养基中仅能维持1天，而不会在转移至诱导培养基后显著丧失分化潜力。在高生长素和低细胞分裂素或低生长素和高细胞分裂素的培养基中初始培养长达2天，可使细胞在转移至诱导培养基后的第三天实现完全分化，并增强一些细胞的早期分化。
