Church D L, Galston A W
Department of Biology, Yale University, New Haven, CT 06511.
Phytochemistry. 1988;27(8):2435-9. doi: 10.1016/0031-9422(88)87008-0.
Mechanically isolated mesophyll cells of Zinnia elegans L. cv Envy differentiate to tracheary elements when cultured in inductive medium containing sufficient auxin and cytokinin. Tracheary element differentiation was induced by the three auxins (alpha-naphthaleneacetic acid, indole-3-acetic acid, and 2,4-dichlorophenoxyacetic acid) and four cytokinins (6-benzyladenine, kinetin, 2-isopentenyladenine and zeatin) tested. Tracheary element formation is inhibited or delayed if the inductive medium is supplemented with an anticytokinin, antiauxin, or inhibitor of auxin transport.
百日草“羡慕”品种机械分离的叶肉细胞，在含有足够生长素和细胞分裂素的诱导培养基中培养时会分化为管状分子。所测试的三种生长素（α-萘乙酸、吲哚-3-乙酸和2,4-二氯苯氧乙酸）和四种细胞分裂素（6-苄基腺嘌呤、激动素、2-异戊烯基腺嘌呤和玉米素）均可诱导管状分子分化。如果在诱导培养基中添加抗细胞分裂素、抗生长素或生长素运输抑制剂，管状分子的形成会受到抑制或延迟。
