Joyce G F
Departments of Chemistry and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037.
Pure Appl Chem. 1993 Jun;65(6):1205-12. doi: 10.1351/pac199365061205.
An RNA-based evolution system was constructed in the laboratory and used to develop RNA enzymes with novel catalytic function. By controlling the nature of the catalytic task that the molecules must perform in order to survive, it is possible to direct the evolving population toward the expression of some desired catalytic behavior. More recently, this system has been coupled to an in vitro translation procedure, raising the possibility of evolving protein enzymes in the laboratory to produce novel proteins with desired catalytic properties. The aim of this line of research is to reduce darwinian evolution, the fundamental process of biology, to a laboratory procedure that can be made to operate in the service of organic synthesis.
在实验室构建了一个基于RNA的进化系统，并用于开发具有新型催化功能的RNA酶。通过控制分子为了存活而必须执行的催化任务的性质，有可能引导进化群体朝着表达某些期望的催化行为发展。最近，该系统已与体外翻译程序相结合，增加了在实验室中进化蛋白质酶以产生具有所需催化特性的新型蛋白质的可能性。这一系列研究的目的是将生物学的基本过程——达尔文进化，简化为一种可用于有机合成的实验室程序。
