Ling V, Assmann S M
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138.
Plant Physiol. 1992;100(2):970-8. doi: 10.1104/pp.100.2.970.
The distribution of calmodulin (CaM) and CaM-binding proteins within Vicia faba was investigated. Both CaM and CaM-binding proteins were found to be differentially distributed among organs, tissues, and protoplast types. CaM levels, on a per protein basis, were found to be the highest in leaf epidermis, containing 3-fold higher levels of CaM than in total leaf. Similarly, guard cell and epidermal cell protoplasts were also found to have higher levels of CaM than mesophyll cell protoplasts. 125I-CaM blot overlay assays were performed to qualitatively examine CaM-binding proteins in these protoplast types as well as in whole tissues and organs. CaM-binding proteins with Mr 52,000, 78,000, and 115,000 were common in all metabolically active plant parts. Unique CaM-binding protein bands were detected in guard cell protoplasts (Mr 39,000, 88,000), stems (Mr 45,000, 60,000, 64,000), and roots (Mr 62,000), suggesting the presence of specialized CaM-dependent processes in these cells and organs.
研究了蚕豆中钙调蛋白（CaM）和钙调蛋白结合蛋白的分布情况。发现CaM和钙调蛋白结合蛋白在不同器官、组织和原生质体类型中呈差异分布。以蛋白质含量为基础，发现叶表皮中的CaM水平最高，其CaM含量比整个叶片中的高3倍。同样，保卫细胞和表皮细胞原生质体中的CaM水平也高于叶肉细胞原生质体。进行了¹²⁵I-CaM印迹覆盖分析，以定性检测这些原生质体类型以及整个组织和器官中的钙调蛋白结合蛋白。分子量为52,000、78,000和115,000的钙调蛋白结合蛋白在所有代谢活跃的植物部分中都很常见。在保卫细胞原生质体（分子量39,000、88,000）、茎（分子量45,000、60,000、64,000）和根（分子量62,000）中检测到独特的钙调蛋白结合蛋白条带，表明这些细胞和器官中存在特殊的钙调蛋白依赖性过程。
