Mac Neil S, Walker S W, Senior H J, Bleehen S S, Tomlinson S
J Invest Dermatol. 1984 Jul;83(1):15-9. doi: 10.1111/1523-1747.ep12261637.
Two drugs known to inhibit the action of calmodulin, prochlorperazine offP) and N-(6-aminohexyl)-5-chloro-1-napthalene sulfonamide (W7), were investigated for their ability to control cell proliferation in murine B16 melanoma cells in culture. PCP and W7 inhibited [3H]thymidine uptake in these cells, 50% inhibition occurring with 13 microM PCP and 40 microM W7. In the presence of relatively high concentrations of fetal calf serum (FCS), cells withstood high concentrations of both drugs (100 microM PCP and 200 microM W7) and showed increased pigment production. Drug-inhibited DNA synthesis could be reversed by the addition of fresh medium containing FCS or by the addition of exogenous pure calmodulin. Extracellular calmodulin itself stimulated DNA synthesis. FCS was found to contain calmodulin-like activity at concentrations that may be relevant to the stimulation of [3H]thymidine uptake by cells in culture.
研究了两种已知可抑制钙调蛋白作用的药物，即氯丙嗪（PCP）和N-（6-氨基己基）-5-氯-1-萘磺酰胺（W7），考察它们对培养的小鼠B16黑色素瘤细胞增殖的控制能力。PCP和W7抑制这些细胞对[3H]胸腺嘧啶核苷的摄取，13μM的PCP和40μM的W7可产生50%的抑制率。在相对高浓度的胎牛血清（FCS）存在下，细胞能耐受高浓度的两种药物（100μM的PCP和200μM的W7），并表现出色素生成增加。添加含FCS的新鲜培养基或添加外源性纯钙调蛋白可逆转药物抑制的DNA合成。细胞外钙调蛋白本身可刺激DNA合成。发现FCS在可能与刺激培养细胞摄取[3H]胸腺嘧啶核苷相关的浓度下具有钙调蛋白样活性。
