Roubein I F, Keup W
Res Commun Chem Pathol Pharmacol. 1975 Apr;10(4):633-40.
The formation of DMI after incubation of IP-C-14 with supernatant fractions prepared from brain and liver of male Wistar rats were studied to determine the sources of DMI in brain tissue. IP and its metabolites were isolated by thin layer chromatography, eluted and counted in a Geiger-Muller counter. A substantial amount of DMI was formed when the liver supernatant fraction was used, whereas the brain fraction did not form DMI even at low level. Under the incubation condition used, about 2.7% of IP was converted non-enzymatically to a DMI-like substance. Based on the results presented it appears that at least most of the DMI reported by other authors to be formed in rat brain after IP administration, reaches the brain via the blood stream.
研究了用雄性Wistar大鼠脑和肝制备的上清液组分与IP-C-14孵育后DMI的形成，以确定脑组织中DMI的来源。通过薄层色谱法分离IP及其代谢物，洗脱后在盖革-米勒计数器中计数。使用肝上清液组分时会形成大量DMI，而脑组分即使在低水平下也不形成DMI。在所使用的孵育条件下，约2.7%的IP非酶促转化为类似DMI的物质。根据所呈现的结果，其他作者报道的IP给药后在大鼠脑中形成的DMI，似乎至少大部分是通过血流到达脑的。
