Microassay of an arylamidase in needle biopsies of human liver.

A method is described for determination of liver arylamidase activity in homogenates of the small amount of human as well as experimental animal tissues (approx. 5-15 mg wet wt.) obtained by needle biopsy. One milligram of human liver contains 90-130 mug protein. The arylamidase activity in normal liver was 0.2 to 0.6 nmoles benzylhydrazine formed/mug protein/hr. The microdetermination of an arylamidase described in this paper is worth in the diagnosis of hepatic injury because of the use of biopsy sample.