Friedman M A, Aggarwal V, Lester G E
Res Commun Chem Pathol Pharmacol. 1975 Jun;11(2):311-8.
Cycloheximide, pactamycin and tenuazonic acid inhibit protein synthesis in eukaryotic cells. It is the purpose of these studies to determine whether these agents would be effective in vivo inhibitors of epidermal DNA synthesis. Single I.P. administration of 1.5 mg/kg cycloheximide, 0, 1 or 3 hours prior to a 45 minute pulse of thymidine-3H inhibited epidermal DNA synthesis by 68%, 63% and 88%, respectively. This response was dose related at 3.75 hours after cycloheximide with no effect at 0.375 mg/kg and maximal inhibition at 1.5 mg/kg. Epidermal DNA synthesis was inhibited 18, 29 and 62% at doses of 1.5, 3.0 and 6.0 mg/kg pactamycin administered 3 hours before thymidine-3H. Similarly, at doses of 70 and 140 mg/kg tenuazonic acid, epidermal DNA synthesis was inhibited by 50% and 60%, respectively. These data suggest the possible use of protein synthesis inhibitors in epidermal disorders.
放线菌酮、密旋霉素和细交链孢菌酮酸可抑制真核细胞中的蛋白质合成。这些研究的目的是确定这些药物是否会成为表皮DNA合成的有效体内抑制剂。在给予3H-胸腺嘧啶核苷45分钟脉冲之前0、1或3小时，腹腔注射1.5mg/kg放线菌酮，分别使表皮DNA合成抑制68%、63%和88%。在放线菌酮给药后3.75小时，这种反应与剂量相关，0.375mg/kg时无作用，1.5mg/kg时抑制作用最大。在给予3H-胸腺嘧啶核苷前3小时，给予1.5、3.0和6.0mg/kg密旋霉素，表皮DNA合成分别被抑制18%、29%和62%。同样，给予70和140mg/kg细交链孢菌酮酸时，表皮DNA合成分别被抑制50%和60%。这些数据表明蛋白质合成抑制剂可能用于治疗表皮疾病。
