Sjödahl R, Tagesson C
Scand J Gastroenterol. 1975;10(5):459-63.
Human gallbladder epithelium was disintegrated to complete loss of microscopic structure. The material obtained - an extract - was incubated at 37 degrees C together with a mixture of lecithin and lysolecithin, phospholipids known to be present in bile. During such incubation a change in the phospholipid composition occurred, indicating that lecithin was converted to lysolechitin. This conversion increased in the presence of CaCl2. Heating the disintegrated epithelium (70 degrees C for 10 min at pH 4) considerably increased the phospholipid conversion. The results are consistent with the presence of phospholipase A activity in human gallbladder epithelium. The possible significance of this activity is discussed in view of the inflammation-mediating activity of lysolecithin in the gallbladder epithelium.
人胆囊上皮细胞解体，微观结构完全丧失。所获得的物质——一种提取物——与卵磷脂和溶血卵磷脂的混合物一起在37℃下孵育，已知这两种磷脂存在于胆汁中。在这种孵育过程中，磷脂组成发生了变化，表明卵磷脂转化为溶血卵磷脂。在氯化钙存在的情况下，这种转化增加。将解体的上皮细胞加热（在pH值为4的条件下于70℃加热10分钟）可显著提高磷脂转化。这些结果与人类胆囊上皮细胞中存在磷脂酶A活性一致。鉴于溶血卵磷脂在胆囊上皮细胞中的炎症介导活性，讨论了这种活性可能的意义。
