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体外培养的大豆胚对温度和光周期的响应

Temperature and photoperiod responses of soybean embryos cultured in vitro.

作者信息

Raper C D, Patterson R P

机构信息

Department of Soil Science, North Carolina State University, Raleigh 27695-7619, USA.

出版信息

Can J Bot. 1986;64:2411-3. doi: 10.1139/b86-320.

DOI:10.1139/b86-320
PMID:11539710
Abstract

Temperature and photoperiod each have direct effects on growth rate of excised embryos of soybean (Glycine max (L.) Merrill). To determine if the effects of photoperiod are altered by temperature, embryos of 'Ransom II' were cultured in vitro at 18, 24, and 30 degrees C under photoperiod durations of 12 and 18 h at an irradiance of 9 W m-2 (700 to 850 nm) and a photosynthetic photon flux density of 58 micromoles m-2 s-1 (400 to 700 nm). Accumulation rates of fresh and dry weight were greater under 18-h than 12-h photoperiods over the entire range of temperature. Water content of the culture embryos was not affected by photoperiod but was greater at 18 and 30 than 24 degrees C. The accumulation rate of dry weight increased from 18 to 26 but declined at 30 degrees C.

摘要

温度和光周期对大豆(Glycine max (L.) Merrill)离体胚的生长速率均有直接影响。为了确定温度是否会改变光周期的影响,将‘兰塞姆二号’的胚在体外于18、24和30摄氏度下培养,光周期时长分别为12小时和18小时,辐照度为9瓦/平方米(700至850纳米),光合光子通量密度为58微摩尔/平方米·秒(400至700纳米)。在整个温度范围内,18小时光周期下的鲜重和干重积累速率均高于12小时光周期。培养胚的含水量不受光周期影响,但在18和30摄氏度时高于24摄氏度。干重积累速率从18摄氏度增加到26摄氏度,但在30摄氏度时下降。

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