Serlin B S, Roux S J
Botany Department, University of Texas at Austin 78712, USA.
Biochim Biophys Acta. 1986;848:372-7. doi: 10.1016/0005-2728(86)90213-6.
Mitochondria extracted from plants that were irradiated with actinic light in vivo have associated with them the chromoprotein, phytochrome. This phytochrome retains its native subunit size of 124 kDa after proteolytic treatment of the mitochondria with trypsin and chymotrypsin. This result suggests that phytochrome is not exposed on the outer surface of the outer mitochondrial membrane. Phytochrome, so protected, is not found to be associated with mitochondria derived from unirradiated plants. The possibility that the photoactivation of phytochrome induces a conformational change in its structure which facilitates its transport into the mitochondrion is discussed.
从体内经光化光照射的植物中提取的线粒体与色素蛋白——光敏色素相关联。在用胰蛋白酶和胰凝乳蛋白酶对线粒体进行蛋白水解处理后，这种光敏色素保留了其124 kDa的天然亚基大小。这一结果表明，光敏色素并未暴露在线粒体外膜的外表面。如此受到保护的光敏色素，在从未经照射的植物中提取的线粒体中未被发现。文中讨论了光敏色素的光激活是否会诱导其结构发生构象变化，从而促进其转运到线粒体中的可能性。
