Palmisano A C, Cronin S E, Des Marais D J
National Aeronautics and Space Administration, Ames Research Center, Moffett Field, CA 94035, USA.
J Microbiol Methods. 1988;8:209-17. doi: 10.1016/0167-7012(88)90003-6.
As assay for lipophilic pigments in phototrophic microbial mat communities using reverse phase-high performance liquid chromatography was developed which allows the separation of 15 carotenoids and chloropigments in a single 30 min program. Lipophilic pigments in a laminated mat from a commercial salina near Laguna Guerrero Negro, Baja California Sur, Mexico reflected their source organisms. Myxoxanthophyll, echinenone, canthaxanthin, and zeaxanthin were derived from cyanobacteria; chlorophyll c, and fucoxanthin from diatoms; chlorophyll a from cyanobacteria and diatoms; bacteriochlorophylls a and c, bacteriophaeophytin a, and gamma-carotene from Chloroflexus spp.; and beta-carotene from a variety of phototrophs. Sensitivity of detection was 0.6-6.1 ng for carotenoids and 1.7-12 ng for most chloropigments. This assay represents a significant improvement over previous analyses of lipophilic pigments in microbial mats and promises to have a wider application to other types of phototrophic communities.
开发了一种使用反相高效液相色谱法分析光合微生物垫群落中亲脂性色素的方法，该方法可在30分钟的单一程序中分离出15种类胡萝卜素和叶绿素。来自墨西哥南下加利福尼亚州格雷罗内格罗湖附近一个商业盐沼的层状垫中的亲脂性色素反映了其来源生物。粘球藻黄素、海胆酮、角黄素和玉米黄质来自蓝细菌；叶绿素c和岩藻黄素来自硅藻；叶绿素a来自蓝细菌和硅藻；细菌叶绿素a和c、细菌脱镁叶绿素a和γ-胡萝卜素来自绿弯菌属；β-胡萝卜素来自多种光合生物。类胡萝卜素的检测灵敏度为0.6-6.1纳克，大多数叶绿素的检测灵敏度为1.7-12纳克。该分析方法相对于以往对微生物垫中亲脂性色素的分析有显著改进，并有望在其他类型的光合群落中得到更广泛的应用。
