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嗜冷玫瑰微球菌菌株的主要类胡萝卜素色素:纯化、结构及其与合成膜的相互作用。

The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain: purification, structure, and interaction with synthetic membranes.

作者信息

Jagannadham M V, Rao V J, Shivaji S

机构信息

Centre for Cellular & Molecular Biology, Hyderabad, India.

出版信息

J Bacteriol. 1991 Dec;173(24):7911-7. doi: 10.1128/jb.173.24.7911-7917.1991.

Abstract

The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain was purified to homogeneity from methanol extracts of dried cells by reverse-phase liquid chromatography and was designated P-3. On the basis of the UV-visible, infrared, mass, and 1H nuclear magnetic resonance spectra of P-3, it was identified as bisdehydro-beta-carotene-2-carboxylic acid. The pigment interacted with synthetic membranes of phosphatidylcholine and dimyristoyl phosphatidylcholine and stabilized the membranes. These results also indicate that P-3 is different from canthaxanthin, the major carotenoid pigment from a mesophilic M. roseus strain.

摘要

从一株嗜冷玫瑰微球菌菌株的干燥细胞甲醇提取物中,通过反相液相色谱法将其主要类胡萝卜素色素纯化至同质,并命名为P-3。根据P-3的紫外可见光谱、红外光谱、质谱和1H核磁共振光谱,确定其为双脱氢-β-胡萝卜素-2-羧酸。该色素与磷脂酰胆碱和二肉豆蔻酰磷脂酰胆碱的合成膜相互作用并稳定了这些膜。这些结果还表明,P-3与来自嗜温玫瑰微球菌菌株的主要类胡萝卜素色素角黄素不同。

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