Applewhite P B, K-Sawhney R, Galston A W
Department of Biology, Yale University, New Haven, CT 06520, USA.
Life Support Biosph Sci. 1997;4(1-2):55-9.
Excised preanthesis flower buds of young Pixie Hybrid tomato plants develop into red ripe fruits in aseptic culture on a modified Murashige-Skoog medium with 3% sucrose at pH 5.8. The addition of certain synthetic auxins (IAA, NAA, IBA), auxin precursors (ISA), or cytokinins (KIN, IPA, ZEA, BAP) to the medium improved the percentage of buds developing into fruits, the weight of the ripe fruits, or both. The best results were obtained by an auxin-cytokinin combination of 10 microM IBA with 1 microM BAP. Storage of the excised buds at low temperature (6 degrees C) for up to 4 weeks before transfer to 27 degrees C caused only minimal deterioration in size and number of the fruit crop. Extension of low-temperature storage to 8 weeks produced smaller fruits that took longer to develop. This system could produce fresh, ripe small tomatoes on a sustained basis for up to 2 months for an isolated environment such as a space vehicle or submarine.
在添加了3%蔗糖、pH值为5.8的改良Murashige-Skoog培养基上进行无菌培养时，摘除的小精灵杂交番茄幼株的花前花蕾会发育成红色成熟果实。向培养基中添加某些合成生长素（吲哚乙酸、萘乙酸、吲哚丁酸）、生长素前体（吲哚硫酸）或细胞分裂素（激动素、异戊烯基腺嘌呤、玉米素、苄氨基嘌呤），可提高发育成果实的花蕾百分比、成熟果实的重量，或两者均提高。生长素与细胞分裂素以10微摩尔吲哚丁酸与1微摩尔苄氨基嘌呤组合时效果最佳。在转移至27℃之前，将摘除的花蕾在低温（6℃）下储存长达4周，只会使果实产量的大小和数量出现极小程度的下降。将低温储存延长至8周会使果实变小，发育所需时间更长。对于诸如太空飞行器或潜艇等孤立环境，该系统可持续长达2个月生产新鲜、成熟的小番茄。
