Ensminger P A, Lipson E D
Department of Physics, Syracuse University, NY 13244-1130, USA.
Planta. 1991 Jul;184(4):506-9. doi: 10.1007/BF00197899.
Null-point action spectra of the light-growth response were measured for three mutants of Phycomyces blakesleeanus (Burgeff) and compared with the action spectrum of the wild type (WT). The action spectrum for L150, a recently isolated "night-blind" mutant, differs from the WT spectrum. The L150 action spectrum has a depression near 450 nm and small alterations in its long-wavelength cutoff, the same spectral regions where its photogravitropism action spectrum is altered. This indicates that the affected gene product influences both phototropism and the light-growth response. For L85, a "hypertropic" (madH) mutant, the light-growth-response action spectrum is very similar to that of WT even though the photogravitropism action spectrum of L85 has been shown previously to be altered in the near-UV region. The affected gene product in this mutant appears to affect phototropic transduction but not light-growth-response transduction. The action spectrum of C110, a "stiff" (madE) mutant, differs significantly from the WT spectrum near 500 nm, the same spectral region where sporangiophores of madE mutants have been shown to have small alterations in second-derivative absorption spectra. This indicates that the madE gene product may be physically associated with a photoreceptor complex, as predicted by system-analysis studies.
测定了布氏梨形孢（Burgeff）三个突变体的光生长反应零位点作用光谱，并与野生型（WT）的作用光谱进行了比较。最近分离出的“夜盲”突变体L150的作用光谱与野生型光谱不同。L150的作用光谱在450nm附近有一个凹陷，其长波长截止有小的变化，其光重力作用光谱改变的也是相同的光谱区域。这表明受影响的基因产物同时影响向光性和光生长反应。对于“超向光性”（madH）突变体L85，尽管之前已表明L85的光重力作用光谱在近紫外区域发生了改变，但其光生长反应作用光谱与野生型非常相似。该突变体中受影响的基因产物似乎影响向光转导，但不影响光生长反应转导。“僵硬”（madE）突变体C110的作用光谱在500nm附近与野生型光谱有显著差异，在该光谱区域，madE突变体的孢子囊柄在二阶导数吸收光谱中显示有小的变化。这表明，如系统分析研究所预测的，madE基因产物可能与光受体复合物在物理上相关联。
