Monie I W, Morgan J R
Teratology. 1975 Apr;11(2):143-51. doi: 10.1002/tera.1420110203.
The formation of cysts has been studied in kidneys removed from day-15 and day-18 rat fetuses and cultured in a mixture of medium 199 (Morgan et al., '50) for periods of up to 15 days. Gas phases of 95% O2 and 5% CO2, and 95% air and 5% CO2, were employed, the latter being considered more satisfactory. In day-15 kidneys cysts formed from the ampullary portions of the collecting tubules after 2 days whereas cysts derived from nephrons were not seen until 9 days of culturing. The latter arose from developing juxtamedullary nephrons. In day-18 kidneys cysts from collecting tubules and nephrons were both present after 3 days of culturing. The latter, in this instance, originated mostly in immature midcortical nephrons, the juxtamedullary mephrons having undergone rapid degeneration. The tubular portion of the nephron seemed to be the primary site of dilatation. Under culture conditions cysts of nephrons thus formed from immature actively developing nephrons and not from those that were mature. Cysts associated with collecting tubules arose from the ampullary (terminal) portions of the latter in both day-15 and day-18 cultured kidneys. The study of cultured mammalian fetal kidneys can provide information on the nature and genesis of renal cysts. It is possible that the same technique also may be helpful for examining the effects of teratogens directly on the kidney.
对从妊娠第15天和第18天的大鼠胎儿摘除的肾脏进行了研究，并在199培养基（Morgan等人，1950年）的混合物中培养长达15天。使用了95%氧气和5%二氧化碳以及95%空气和5%二氧化碳的气相，后者被认为更令人满意。在妊娠第15天的肾脏中，培养2天后，集合小管的壶腹部形成囊肿，而直到培养9天后才见到源自肾单位的囊肿。后者起源于正在发育的近髓肾单位。在妊娠第18天的肾脏中，培养3天后，集合小管和肾单位的囊肿都出现了。在这种情况下，后者主要起源于未成熟的皮质中部肾单位，近髓肾单位已迅速退化。肾单位的管状部分似乎是扩张的主要部位。在培养条件下，这样形成的肾单位囊肿来自未成熟的、正在积极发育的肾单位，而不是成熟的肾单位。在妊娠第15天和第18天培养的肾脏中，与集合小管相关的囊肿都起源于后者的壶腹部（末端）部分。对培养的哺乳动物胎儿肾脏的研究可以提供关于肾囊肿的性质和发生的信息。同样的技术也可能有助于直接研究致畸剂对肾脏的影响。
