Iuzhakov V V, Khavinson V Kh, Kvetnoĭ I M, Fomina N K, Kuznetsova M N
Medical Center for Radiological Research, Russian Academy of Medical Sciences, Obninsk.
Vopr Onkol. 2001;47(3):328-34.
The study was concerned with growth of sarcoma M-1 and basic morphological characteristics of proliferative activity of cells of this strain as well as apoptosis of cells at different stages of tumor progression in rats before and after a single gamma irradiation at 30 Gy. At the parenchymal periphery which determines tumor growth, the PCNA index of proliferating cells was 76.5%; spontaneous cell death--0.28%. During post-irradiation period, the sarcoma PCNA index fell to 62.3% while the apoptotic index rose five-fold. These findings support the concept of radiation-induced apoptosis being a major pathogenetic factor responsible for effectiveness of radiotherapy of tumors. Indirect evidence on PCNA immunostaining suggested that synthesis of this cyclin is sensitive to the level of oxygen input in the cell, yet it offers sufficient resistance to gamma-radiation.
该研究关注肉瘤M-1的生长、该菌株细胞增殖活性的基本形态学特征，以及大鼠在30 Gy单次γ射线照射前后肿瘤进展不同阶段细胞的凋亡情况。在决定肿瘤生长的实质周边，增殖细胞的PCNA指数为76.5%；自发细胞死亡为0.28%。在照射后期间，肉瘤的PCNA指数降至62.3%，而凋亡指数上升了五倍。这些发现支持辐射诱导凋亡是肿瘤放射治疗有效性的主要致病因素这一概念。PCNA免疫染色的间接证据表明，这种细胞周期蛋白的合成对细胞内氧气输入水平敏感，但它对γ射线具有足够的抗性。
